노화 생쥐의 복부지방에서 수영운동에 의한 AMPK와 GLUT4 단백질 변화 = Changes of AMPK & GLUT-4 Protein Expression by Swim Exercise in Fat Tissue of Ageing Mice

다국어 초록 (Multilingual Abstract)

The present study was designed to examine the protein changes of AMPK and GLUT-4 by swim exercise in fat tissue of aging mice. We performed aerobics exercise by swimming, measured body weight(BW)and measured blood glucose(BG), and performed Oil-Red stain for adipocyte and western blot for AMPK and GLUT-4 protein in fat tissue. SWV(n=30) mice at age of 2 months and 12months old were divided into 3 groups as follows: (1) Old control group (n=10, O-Con), (2) Young control group(n=10, Y-Con) (3) Old+exercise group (n=10, O-Ex). The swimming exercise was performed 5 days /week with duration of 40minutes for 8weeks. BW and BG were measured once per week. Results showed that the BW of O-Con group was is higher than other groups, interestingly, BW of Old-Ex was significantly decreased (p<.05, p<.001) from 2nd. BG of O-Ex groups showed markedly decreased (p<.05, p<.001) from 2 weeks after exercise and continuously lower than other groups. Retroperitoneal fat fed weight of O-Ex group was significantly decreased (p<.001) and adipocyte size of O-Con group was bigger than other groups, but O-Ex group smaller sized adipocyte than other groups. Particulary, AMPK and GLUT-4 which are related with lipid metabolism was markedly inhibited compared to Y-Con group, whereas O-Ex group showed increased of AMPK and GLUT-4 protein expression than other groups. These results suggest that swim exercise for 8 week should inhibit adipocyte hypertrophy by controling the BW and decreasing retroperitoneal fat fed through AMPK protein expression and controling protective of BG the by decreasing the BG through GLUT-4 protein expression in fat tissue of aging mice. Thus, exercise may be helpful to improvement of lipid metabolism function and may be protective effect from lipid metabolism disease.

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