C2C12 myotube에서 IGF-I이 엔드로젠 수용체의 유전자 발현 및 활성화에 미치는 영향(결과보고서)

다국어 초록 (Multilingual Abstract)

The modulating effect of IGF-I on the regulation of AR gene expression and activation in skeletal muscle cells remains poorly understood. In this study, the effects of IGF-I treatment on AR induction and activation in the absence of AR ligands were examined. Differentiating C2C12 cells were treated with different concentrations (0-250 ng/ml) of IGF-I or for various periods of time (0-60 min) of 250 ng/ml IGF-I. Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent increase in total AR and phosphorylated AR (Ser 213). IGF-I treatment also led to significantly increased AR mRNA expression when compared with the control. The levels of skeletal ？-actin and myogenin mRNA, known target genes of AR, were also significantly upregulated after 5 or 10 min of treatment with IGF-I. Confocal images revealed that IGF-I stimulated nuclear localization of AR in the absence of ligands. In addition, an electrophoretic mobility shift assay indicated that IGF-I stimulated the AR DNA binding activity in a time-dependent manner. The present results suggest that IGF-I stimulates the expression and activation of AR by ligand-independent mechanism in differentiating C2C12 mouse skeletal muscle cells.

국문 초록 (Abstract)

Ⅰ.연구목적 및 방법
1. 연구의 필요성 및 목적
2. 연구 방법
Ⅱ. 연구 결과
Ⅲ. 연구결과 활용계획
1. 연구결과 활용계획
2. 연구성과
Ⅳ. 참고문헌