High Plasticity of the Gut Microbiome and Muscle Metabolome of Chinese Mitten Crab (Eriocheir sinensis) in Diverse Environments = High Plasticity of the Gut Microbiome and Muscle Metabolome of Chinese Mitten Crab (Eriocheir sinensis) in Diverse Environments

다국어 초록 (Multilingual Abstract)

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab (Eriocheir sinensis) survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of E. sinensis have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on E. sinensis collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of E. sinensis when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.

참고문헌 (Reference)

1 Edgar RC, "UPARSE : highly accurate OTU sequences from microbial amplicon reads" 10 : 996-998, 2013

2 Herborg LM, "The invasion of the Chinese mitten crab(Eriocheir sinensis)in the United Kingdom and its comparison to continental Europe" 7 : 959-968, 2005

3 Sommer F, "The gut microbiota modulates energy metabolism in the hibernating brown bear Ursus arctos" 14 : 1655-1661, 2016

4 Li X, "The effect of stocking density of Chinese mitten crab Eriocheir sinensis on rice and crab seed yields in rice–crab culture systems" 273 : 487-493, 2007

5 Saito Y, "The Huanghe(Yellow River)and Changjiang(Yangtze River)deltas : a review on their characteristics, evolution and sediment discharge during the Holocene" 41 : 219-231, 2001

6 Cheng Q, "Study on genetic variation and adaptive evolution from the native and colonized populations of Chinese mitten crab(in Chinese)" 22 : 161-167, 2013

7 Rudi K, "Stable core gut microbiota across the freshwater-to-saltwater transition for farmed Atlantic Salmon" 84 : e01974-17, 2018

8 Chen L, "Spatial variations of macrozoobenthos and sediment nutrients in Lake Yangcheng : Emphasis on effect of pen culture of Chinese mitten crab" 37 : 118-129, 2015

9 Jarak I, "Response to dietary carbohydrates in European seabass(Dicentrarchus labrax)muscle tissue as revealed by NMR-based metabolomics" 14 : 95-, 2018

10 Wang J, "Resolving the genetic paradox of invasions : preadapted genomes and postintroduction hybridization of bigheaded carps in the Mississippi River Basin" 13 : 263-277, 2020

11 Caporaso JG, "QIIME allows analysis of highthroughput community sequencing data" 7 : 335-336, 2010

12 Shin N-R, "Proteobacteria : microbial signature of dysbiosis in gut microbiota" 33 : 496-503, 2015

13 Langille MGI, "Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences" 31 : 814-821, 2013

14 Kakumanu ML, "Overlapping community compositions of gut and fecal microbiomes in labreared and field-collected German cockroaches" 84 : e01037-01018, 2018

15 Martínez-Mota R, "Natural diets promote retention of the native gut microbiota in captive rodents" 14 : 67-78, 2019

16 Wang Q, "Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy" 73 : 5261-5267, 2007

17 Wang C, "Molecular genetic structure and evolution in native and colonized populations of the Chinese mitten crab, Eriocheir sinensis" 11 : 389-399, 2009

18 Sun Y, "Microbiota comparison in the intestine of juvenile Chinese mitten crab Eriocheir sinensis fed different diets" 515 : 734518-, 2020

19 Hollywood K, "Metabolomics : current technologies and future trends" 6 : 4716-4723, 2006

20 Johnson CH, "Metabolomics : beyond biomarkers and towards mechanisms" 17 : 451-, 2016

21 Schloss PD, "Introducing mothur : open-source, platformindependent, community-supported software for describing and comparing microbial communities" 75 : 7537-7541, 2009

22 Tao H, "Intestinal microbiome affects the distinctive flavor of Chinese mitten crabs in commercial farms" 483 : 38-45, 2018

23 Singh P, "Intestinal microbial communities associated with acute enteric infections and disease recovery" 3 : 45-, 2015

24 Buford TW, "International society of sports nutrition position stand : creatine supplementation and exercise" 4 : 6-, 2007

25 Chen C-Y, "Habitat and indigenous gut microbes contribute to the plasticity of gut microbiome in oriental river prawn during rapid environmental change" 12 : e0181427-, 2017

26 Wishart DS, "HMDB 4.0: the human metabolome database for 2018" 46 : D608-D617, 2017

27 Chevalier C, "Gut microbiota orchestrates energy homeostasis during cold" 163 : 1360-1374, 2015

28 Vijayakumar M. Malathi, "Gut Bacterial Diversity of Insecticide-Susceptible and -Resistant Nymphs of the Brown Planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) and Elucidation of Their Putative Functional Roles" 한국미생물·생명공학회 28 (28): 976-986, 2018

29 Wang J, "Genetic improvement and breeding practices for Chinese mitten crab, Eriocheir sinensis" 49 : 292-301, 2018

30 Wang J, "Genetic improvement and breeding practices for Chinese mitten crab, Eriocheir sinensis" 49 : 292-301, 2018

31 Chen S, "Fastp : an ultra-fast all-in-one FASTQ preprocessor" 34 : i884-, 2018

32 Magoč T, "FLASH : fast length adjustment of short reads to improve genome assemblies" 27 : 2957-2963, 2011

33 Liu H, "Effects of hairy crab breeding on drinking water quality in a shallow lake" 662 : 48-56, 2019

34 Lu J, "Effect of fermented corn-soybean meal on serum immunity, the expression of genes related to gut immunity, gut microbiota, and bacterial metabolites in grower-finisher pigs" 10 : 2620-, 2019

35 Wang W, "Ecological Aquaculture of Chinese mitten crab" China Agriculture Press 30-, 2013

36 Alberdi A, "Do vertebrate gut metagenomes confer rapid ecological adaptation?" 31 : 689-699, 2016

37 Naas AE, "Do rumen bcteroidetes utilize an alternative mechanism for cellulose degradation?" 5 : e01401-e01414, 2014

38 Smith CCR, "Dietary input of microbes and host genetic variation shape among-population differences in stickleback gut microbiota" 9 : 2515-2526, 2015

39 Gao Y-M, "Deep insights into gut microbiota in four carnivorous coral reef fishes from the South China Sea" 8 : 426-, 2020

40 Kong L, "Comparison of non-volatile compounds and sensory characteristics of Chinese mitten crabs (Eriocheir sinensis) reared in lakes and ponds: potential environmental factors" 364-365 : 96-102, 2012

41 Wang S, "Comparison of flavour qualities of three sourced Eriocheir sinensis" 200 : 24-31, 2016

42 Yang Fan, "Comparison of Microbial Diversity and Composition in the Jejunum and Colon of Alcohol-Dependent Rats" 한국미생물·생명공학회 28 (28): 1883-1895, 2018

43 Li K, "Comparative analysis of gut microbiota of native Tibetan and Han populations living at different altitudes" 11 : e0155863-, 2016

44 García A, "Capillary electrophoresis mass spectrometry as a tool for untargeted metabolomics" 9 : 99-130, 2016

45 Rothman JA, "Cadmium and selenate exposure affects the honey bee microbiome and metabolome, and bee-associated bacteria show potential for bioaccumulation" 85 : e01411-19, 2019

46 Fox RJ, "Beyond buying time : the role of plasticity in phenotypic adaptation to rapid environmental change" 374 : 20180174-, 2019

47 Thévenot EA, "Analysis of the human adult urinary metabolome variations with age, body mass index, and gender by implementing a comprehensive workflow for univariate and OPLS statistical analyses" 14 : 3322-3335, 2015

48 Chevin L-M, "Adaptation, plasticity, and extinction in a changing environment : Towards a predictive theory" 8 : e1000357-, 2010

49 Alvares TS, "Acute L-arginine supplementation increases muscle blood volume but not strength performance" 37 : 115-126, 2012

50 Smith JL, "A review of cyanobacterial odorous and bioactive metabolites : Impacts and management alternatives in aquaculture" 280 : 5-20, 2008