<i>Duganella</i> <i>sacchari</i> sp. nov. and <i>Duganella</i> <i>radicis</i> sp. nov., two novel species isolated from rhizosphere of field-grown sugar cane

다국어 초록 (Multilingual Abstract)

<P>Two strains, designated Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP>, were isolated from rhizosphere soil and rhizoplane of field-grown sugar cane clone Co86032. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class <I>Betaproteobacteria</I>, their closest relatives being <I>Pseudoduganella violaceinigra</I> and <I>Duganella zoogloeoides</I> with 16S rRNA gene sequence pairwise similarities of 96.4–97.2 % to the two novel strains. Strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> shared a 16S rRNA gene sequence similarity value of 97.6 %. Cells of the two strains were Gram-reaction-negative, aerobic, motile and rod-shaped. Ubiquinone (Q-8) was the respiratory quinone and the predominant polar lipids consisted of phosphatidylglycerol and phosphatidylethanolamine. The main cellular fatty acids were C<SUB>16 : 0</SUB>, C<SUB>16 : 1</SUB>ω7<I>c</I>/iso-C<SUB>15 : 0</SUB> 2-OH, C<SUB>17 : 0</SUB> cyclo, C<SUB>10 : 0</SUB> 3-OH and C<SUB>12 : 0</SUB>. The DNA G<I>+</I>C content of the genomic DNA was 56.4 mol% for strain Sac-22<SUP>T</SUP> and 54.9 mol% for strain Sac-41<SUP>T</SUP>. Based on the results of 16S rRNA gene sequence analysis and physiological and biochemical characterization, that differentiated strains Sac-22<SUP>T</SUP> and Sac-41<SUP>T</SUP> from all recognized species of the genus <I>Duganella</I>, it was concluded that strains represent two novel species in the genus <I>Duganella</I> for which the names <I>Duganella</I> <I>sacchari</I> sp. nov. (type strain Sac-22<SUP>T</SUP> = KCTC 22381<SUP>T</SUP> = NCIMB 14475<SUP>T</SUP>) and <I>Duganella</I> <I>radicis</I> sp. nov. (type strain Sac-41<SUP>T</SUP> = KCTC 22382<SUP>T</SUP> = NCIMB 14476<SUP>T</SUP>) are proposed.</P>