통계적 방법을 이용한 항산화활성 증진 최적 식물추출물 조합 조건

다국어 초록 (Multilingual Abstract)

This study was conducted to develop plant extracts with antioxidant activities as potential animal feed additives by using statistical models. A total of 10 different plant extracts (Leonurus japonicus Houtt., Morus alba L. leaves, Eucommia ulmoides Oliv., Taraxacum platycarpum Dahlst., Kalopanax pictus Nakai, Saururus chinensisBaill., Cudrania tricuspidata (Carriere) Bureau ex Lavallee, Diospyros kaki Thunb. leaves, Hovenia dulcis Thunb., and Acanthopanax senticosus (Rupr. & Maxim.) Harms) were prepared using a water extraction method, and their total polyphenol, and flavonoid levels and antioxidant activities were determined. The antioxidant activities of the plant extracts, when all the extracts were mixed, were monitored using the Plackett-Burman design, which is a fractional factorial experimental design. Positive effects (p<0.05) were observed in M. alba, D. kaki leaves and A. senticosus leaves. The optimum ratio of the 3 plant extracts was investigated using a mixture design, and the root mean square prediction error was used in the calculation of theresponse. The optimum ratio of M. alba : D. kaki leaves : A. senticosus leaves appeared to be 0.44 : 0.28 : 0.28.

참고문헌 (Reference)

1 박해룡, "혼합물실험설계법에 의한 라면 밀가루 혼합비의 최적화" 한국산업식품공학회 15 (15): 297-304, 2011

2 이승은, "헛개나무의 Angiotensin 전환 효소 저해 및 항산화 활성" 한국약용작물학회 12 (12): 79-84, 2004

3 장민선, "천연 갈변저해제로서의 민들레 및 감국 추출물의 항산화 및 갈변억제 효과" 한국식품저장유통학회 18 (18): 584-589, 2011

4 강선영, "산란계에 천연 항산화원으로서 가시오갈피 및 두충 급여가 체내 항산화 작용에 미치는 영향" 한국가금학회 37 (37): 15-21, 2010

5 이상무, "사료 내 엄나무 첨가 급여가 육계의 생산성 및 계육의 지방산 조성에 미치는 영향" 한국축산식품학회 30 (30): 305-312, 2010

6 전예숙, "뽕잎 추출물의 항산화 효과와 항산화 성분 분리 및 동정에 관한 연구" 한국식품영양학회 24 (24): 94-100, 2011

7 정승일, "감잎의 성장시기별 항산화 효과" 한국생약학회 41 (41): 255-263, 2010

8 김선제, "益母草와 茺蔚子의 高脂血症 억제 효능" 대한본초학회 25 (25): 73-80, 2010

9 Zhang, Y. J., "Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)" 13 : 103-110, 2012

10 Chaves, A. V., "Evaluation of cornell net carbohydrate and protein system predictions of milk production, intake and liveweight change of grazing dairy cows fed contrast silages" 144 : 85-, 2006

11 Juan, M. Y., "Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 14715" 27 : 586-591, 2010

12 Oliveira, R. A., "Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves" 93 : 4280-4291, 2010

13 Vanaja, K., "Design of experiments: Concept and applications of Plackett Burman Design" 24 : 1-23, 2007

14 Hras, A., "Comparison of antioxidative and synergistic effects of rosemary extract with α-tocopherol, ascorbyl palmitate and citric acid in sunflower oil" 72 : 229-233, 2000

15 Lee, B. W., "Antioxidant and cytotoxic activities of xanthones from Cudrania tricuspidata" 15 : 5548-5552, 2005

16 Kayano, S. I., "Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist" 50 : 3708-3712, 2002

17 Lee, W. S., "Antioxidant activities of a new lignan and a neolignan from Saururus chinensis" 14 : 5623-5628, 2004