장기간 계대배양 된 장미 배발생 캘러스로부터 식물체 재분화 및 비정형체로부터 새로운 배발생캘러스 재생 = New embryogenesis from atypical bodies and plant regeneration from long-term subcultured embryogenic callus in rose

다국어 초록 (Multilingual Abstract)

Long-term subcultured rose embryogenic calluses,which had been maintained for more than 5 to 6 years sincethe first embryogenesis from calluses induced from in vitroroots of rose, were identified as potential material for thedevelopment of transgenic plants. The first embryogeniccalluses from ‘Sweet Yellow’ and two breeding lines (KR056002and KR056006) were obtained in 2007 and 2009, respectively.
Subsequently, we found that plants regenerated from longtermembryogenic calluses (LEC). Whereas the LEC from‘Sweet Yellow’ takes 3 to 4 months to regenerate plants,those of the two breeding lines take 4 to 5 months. Thisperiod of time is the same as that taken for plants to regeneratefrom the first embryogenic callus. New embryogenesis wasobserved from atypical bodies (ABs) that appeared duringthe process of long-term subculture. We found that it ispossible to use the AB as a material for new embryogenesis.

참고문헌 (Reference)

1 이수영, "배발생 캘러스를 이용한 아그로박테리움 매개형질전환 장미 식물체 획득" 한국식물생명공학회 37 (37): 511-516, 2010

2 Lee SY, "safety white paper 2013" KRIBB(eds)Biosafety white paper 260-269, 2013

3 Li, X, "Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa" 159 : 313-319, 2002

4 Dohm A, "Somatic embryogenesis in roses" 547 : 341-347, 2001

5 Lambé P, "Somatic embryogenesis in pearl millet (Pennisetum glaucum): Strategies to reduce genotype limitation and to maintain long-term totipotency" 55 : 23-29, 1999

6 Kim CK, "Somatic embryogenesis from in vitro grown leaf explants of Rosa hybrida L" 5 : 169-172, 2003

7 Lee SY, "Somatic embryogenesis and shoot development in Rosa hybrida L" 870 : 219-225, 2010

8 Marchant R, "Somatic embryogenesis and plant regeneration in floribunda rose (Roas hybrida L.) cvs. Trumpeter and Glad Tidings" 120 : 95-105, 1996

9 Motoike SY, "Somatic embryogenesis and long term maintenance of embryogenic lines lines from fox grapes" 66 : 121-131, 2001

10 Brisibe EA, "Regulation of somatic embryogenesis in long-term callus-cultures of sugarcane (Saccharum officinarum L.)" 126 : 301-307, 1994

11 Firozabody E, "Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissues" 12 : 609-613, 1994

12 Zakizadeh H, "Regeneration of miniature potted rose (Rosa hybrida L.) via somatic embryogenesis" 73 : 111-117, 2008

13 Kamo K, "Regeneration from long-term embryogenic callus of the Rosa hybrida cultivar Kardinal" 41 : 32-36, 2005

14 이수영, "Production of Somatic Embryo and Transgenic Plants Derived from Breeding Lines of Rosa hybrida L." 한국원예학회 54 (54): 172-176, 2013

15 Dubois LAM, "Preliminary report on the direct regeneration of adventitious buds on leaf explants of in vivo grown glasshouse rose cultivars" 60 : 249-253, 1995

16 Hsia C, "Organogenesis and somatic embryogenesis in callus of Rosa hybrida and Rosa chinensis minima" 44 : 1-6, 1996

17 Wang HC, "Morphogenetic routes of long-term embryogenic callus culture of Areca catechu" 54 : 1-5, 2010

18 Hua YW, "Micropropagation of self-rooting juvenile clones by secondary somatic embryogenesis in Hevea brasiliensis" 129 : 202-207, 2010

19 Schenk RU, "Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures" 50 : 199-204, 1972

20 Breton D, "Long-term subculture randomly affects morphology and subsequent maturation of early somatic embryos in maritime pine" 87 : 95-108, 2006

21 Borrelli GM, "Long-term optimized ermbryogenic cultures in drum-wheat (Triticum drum Desf)" 10 : 296-299, 1991

22 Liu L, "Long-term cultured callus and the effect factor of high-frequency plantlet regeneration and somatic embryogenesis maintenance in Zoysia japonica" 45 : 673-680, 2009

23 이수영, "In vitro multiple shoot proliferation and plant regeneration in rose (Rosa hybrida L.)" 한국식물생명공학회 35 (35): 223-228, 2008

24 Tanaka Y, "Flower color modification by genetic engineering" 9 : 2009

25 Ibrahim R, "Factors controlling high efficiency adventitious bud formation and plant regeneration in vitro leaf explants of roses (Rosa hybrida L.)" 88 : 41-57, 2001

26 Pati PK, "Direct shoot regeneration from leaf explants of Rosa damascena Mill" 40 : 192-195, 2004

27 Tian LN, "Continuous long-term somatic embryogenesis in alfalfa" 38 : 279-284, 2002

28 Chai M, "Callus induction, plant regeneration, and long-term maintenance of embryogenic cultures in Zoysia matrella [L.] Merr" 104 : 187-192, 2011