북방전복, Haliotis discus hannai 3배체의 생식능력 = Maturity and Spawning of the Triploid Pacific Abalone, Haliotis discus hannai

국문 초록 (Abstract)

참전복의 배수체별 생식소 조직 관찰에서 2배체 난소의 경우에는 성숙기를 거쳐 방출기로 들어가고 있는 상태이며, 2배체 정소의 경우에는 방출기의 상태였다, 이에 비해 3배체 전복의 생식소는 발달이 억제되어 난소는 성장기 혹은 성숙기의 상태이고, 정소는 성숙기의 단계에 머물고 있다. 따라서 전복은완전한 불임이 되는 것은 아니고 어느 정도 성숙이 억제된다고생각된다.
3배체 참전복의 산란 유발 실험에서 산란 유발에 반응한 개체는 2배체는 암수 전부 반응해서 100%의 산란 유발율을 나타냈고, 3배체는 암컷 50%, 수컷은 25%의 산란 유발율을 나타냈다.
산란된 알과 정자를 배수체별로 조사한 결과, 2배체의 정자는 장경 17.47 μm, 단경 11.40 μm 및 정자의 길이103.94 μm이었던 데 비해 3배체 정자는 장경 10.86μm, 단경 8.47 μm 및 정자의 길이 85.06 μm로서 2배체 정자가 3배체 정자보다 장경, 단경, 정자의 길이 모두에서 유의적으로길었다 (P < 0.05). 알의 직경은 2배체가 199.50 μm, 3배체가 192.50 μm로서 2배체가 컸으나 유의한 차는 없었다( P > 0.05).
배수체별 교배 실험에서 2배체 × 2배체는 정상적으로 수정되어서 발생을 했으나 2배체 알 × 3배체 정자와 3배체 알 ×2배체 정자의 경우에는 수정이 되지 않았다.

다국어 초록 (Multilingual Abstract)

The gonadal development of triploid and diploid Pacific abalones, Haliotis discus hannai was histologically investigated in spawning season. Diploid abalones had matured oocytes and spermatozoa, but most triploid had spermatocytes or developing oocytes that was slightly retarded in gonadal development compared to diploid abalones. In spawning experiment of triploid and diploid abalones, spawning rates of diploid male and female were 100%, but those of triploid female was 50% and male was 25% respectively. Investigation of spawned abalone eggs and spermatozoa revealed that length of diploid sperms head were 17.47 μm, breadth of head were 10.31 μm, length of spermatozoa were 130.72 μm, but those of triploid spermatozoa were 11.83 μm, 7.89 μm and 103.36 μm respectively. Triploid spermatozoa were significantly small to diploid spermatozoa (p < 0.05). The eggs of diploid and triploid were not different in size. The cross experiment between oocytes produced by triploids and spermatozoa by diploids (3n × 2n cross) revealed that no fertilization were occurred, and 2n × 3n cross also revealed same result.

참고문헌 (Reference)

1 지영주, "북방전복, Haliotis discus hannai 3배체의 세포유전학적 연구" 한국패류학회 28 (28): 37-43, 2012

2 赤繁 悟, "三倍體マガキにおける性成熟の特徵" 18 : 41-51, 1992

3 Kim, D. S., "Triploidy Production of Nile Tilapia, Oreochromis niloticus" 3 : 135-144, 1990

4 Arai, K., "Triplodization of the Pacific abalone with Temperature and Pressure treatments" 52 : 417-422, 1986

5 Tomita, K., "The maturation of the testes of the abalone, Haliotis discus hannai INO, in Rebun island, Hokkaido, Japan" 9 : 56-61, 1968

6 Tomita, K., "The maturation of the ovaries of the abalone, Haliotis discus hannai INO, in Rebun island, Hokkaido, Japan" 7 : 1-7, 1967

7 Kikuchi, S., "Technical study on artificial spawning of abalone, genus Haliotis Ⅰ: Relation between water temperature and advancing sexual maturity of Haliotis discus hannai Ino" 33 : 69-78, 1974

8 Kikuchi, S., "Technical study on artificial spawning of abalone, genus Haliotis II: Efect of irradiated sea water with ultraviolet rays on inducing to spawn" 33 : 79-86, 1974

9 Yamazaki, F., "Sex control and manipulation in fish" 33 : 329-354, 1983

10 Lincoln, R. F., "Production of all-female triploid rainbow trout" 30 : 375-, 1983

11 Stanley, J. G., "Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B" 23 : 1-10, 1981

12 Beaumont, A. R., "Ploidy manipulation in molluscan shellfish: a review" 10 : 1-18, 1991

13 Jee, Y. J., "Induction of triploid abalone, Haliotis discus hannai, and Its biological characteristics Ⅲ. Growth and Survival rate of Triploid abalone" 10 : 123-131, 1997

14 Jee, Y. J., "Induction of triploid abalone, Haliotis discus hannai, and Its biological characteristics Ⅰ. Induction of Triploid Abalone" 8 : 159-170, 1995

15 Yang, H. S., "Induction of polyploidy and embryonic development of the abalone, Haliotis diversicolor, with temperature treatment" 14 : 139-147, 1998

16 Tabarini, C. L., "Induced Triploidy in the bay Scallop, Argopecten irradians, and its effect on growth and gametogenesis" 42 : 151-160, 1984

17 Chung, S. C., "Indoor Tank Culture of the Abalone Haliotis discus hannai, Ⅰ. Effect of tank shape and stocking on the growth of young abalone" 7 : 9-20, 1994

18 Chevassus, B., "Hybridization in fish" 33 : 245-262, 1983

19 Okumura, S., "Highly efficient induction of triploid Pacific abalone Haliotis discus hannai by caffeine treatment" 73 : 237-243, 2007

20 Akashige, S., "Growth, Survival, and Glycogen Content of Triploid Pacific Oyster Crassostrea gigas in the Waters of Hiroshima, Japan" 58 : 1063-1071, 1992

21 Komaru, A., "Gametogenesis of triploid Japanese pearl oyster, Pinctada fucata martensii, In "Advances in Invertebrate Reproduction 5"" Elsevier Science Publisher B. V 461-468, 1990

22 Allen, S. K. Jr., "Gametogenesis in three species of triploid shell fish: Mya arenaria, Crassostrea gigas and Crassostrea virginica. Selection, and Hybridization and Genetic Engineering in Aquaculture Vol Ⅱ" Heeneman 207-217, 1987

23 Komaru, A., "Gametogenesis and Growth of Induced Triploid Scallops Chlamys nobilis" 55 : 447-452, 1989

24 Lee, T. Y., "Gamatogenesis and reproductive cycle of abalones. Publ" 7 (7): 21-50, 1974

25 Allen, S. K. Jr., "Cytological evaluation of the likelihood that triploid grass carp with reproduce" 115 : 841-848, 1986

26 Dunstan, G. A., "Culture of triploid greenlip abalone (Haliotis laevigata Donovan) to market size: Commercial implications" 271 : 130-141, 2007

27 Johnson, O. W., "Comparative Growth and Development of Diploid and Triploid Coho Salmon, Oncorhynchus kisutch" 57 : 329-, 1986

28 Taniguchi, N., "Color, growth and maturation in ploidy-manipulated fancy carp" 57 : 321-328, 1986

29 Yang, H. S., "Blocking polar body with cytochalasin B in the fertilized eggs of the small abalone, Haliotis diversicolor supertexta (Lischke), and the development and ploidy of the resultant embryos" 29 : 775-783, 1998

30 Komaru, A., "Better growth and sterility in the induced triploid scallop, Chlamys nobilis and the Japanese pearl oyster, Pinctada fucata martensii" 5-, 1994

31 Allen, S. K. Jr., "Abnormal gametogenesis and Sex ratio in triploid soft-shell clams (Mya arenaria)" 170 : 198-210, 1986

32 지영주, "3배체 참전복, Haliotis discus hannai의 식품 성분에 대한 연구" 한국수산과학회 41 (41): 452-457, 2008