Differential diagnosis of <i>Taenia asiatica</i> using multiplex PCR

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P><P><I>Taenia asiatica</I> and <I>T. saginata</I> are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish <I>T. asiatica</I> infections from infection with other human <I>Taenia</I> tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the <I>cox1</I> gene. Fifty-two samples were analyzed in this study, comprising 20 samples of <I>T. asiatica</I> genomic DNA from China, Korea, and the Philippines; 24 samples of <I>T. saginata</I> from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of <I>T. solium</I> from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the <I>cox1</I> gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing <I>T. asiatica</I>, <I>T. saginata</I>, and <I>T. solium</I> based on 706-, 629-, and 474-bp bands.</P>