IAPV내의 capsid protein gene의 검출을 위한 RT-LAMP 법의 개발

다국어 초록 (Multilingual Abstract)

Israel acute paralysis virus (IAPV) was reported as a virus of colony collapse disorder (CCD) in honeybee. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay allows one-step detection of gene amplification by simple analysis and requires only a simple incubator, such as water bath providing a constant temperature. RT-LAMP method was applied for simple and rapid detection of IAPV. This method was named IAPV-RT-LAMP. The four primers named IAPV-VP2-F3/B3/FIP/BIP were designed for specific amplification of VP2 capsid protein gene in IAPV. Especially, total diagnosis time was reduced until 40 miuntes including reverse transcription reaction from RNA stage. After reaction, LAMP products added fluorescent dye, SYBR Green I and GeneFinder™ were observed with naked eye under daylight without electrophoresis. IAPV-RT-LAMP is expected to be an importance diagnostic tool for detection of IAPV both in laboratory and in field.

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