Henriciella marina gen. nov., sp. nov., a Novel Member of the Family Hyphomonadaceae Isolated from the East Sea

다국어 초록 (Multilingual Abstract)

A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a polyphasictaxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNAgene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, andstrictly aerobic. Strain Iso4T grew optimally at 20°C in the presence of 1~2% (w/v) NaCl and at pH 6.9~7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1 ω7c (53.5%),C17:1 ω5c (11.7%), C17:1 ω6c (8.1%), C16:0 (7.8%), C17:0 (4.8%), C15:0 (2.9%), and C16:1 ω5c (2.2%). The DNAG+C content of strain Iso4T was 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencesshowed that strain Iso4T formed a monophyletic clade in the family Hyphomonadaceae, supported by highbootstrap value and was most closely related to the genus Hyphomonas (92~94%), a member of marinebacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the family Hyphomonadaceae, for which the name Henriciella gen.
nov., sp. nov. is proposed. The type strain is Iso4T (=KCTC 12513T =DSM 19595T =JCM 15116T).

참고문헌 (Reference)

1 Abraham, W.R, "Woodsholeamaritima gen. nov., sp. nov., a marine bacterium with a lowdiversity of polar lipids" 54 : 1227-1234, 2004

2 Saitou, N, "The neighbor-joining method – a newmethod for reconstructing phylogenetic trees" 4 : 406-425, 1987

3 Lee, K.B, "The hierarchical system of the ‘Alphaproteobacteria’: descriptionof Hyphomonadaceae fam. nov., Xanthobacteraceaefam. nov. and Erythrobacteraceae fam. nov" 55 : 1907-1919, 2005

4 Thompson, J.D, "The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided byquality analysis tools" 25 : 4876-4882, 1997

5 Schlesner, H., "Taxonomic and phylogenetic studies on a newtaxon of budding, hyphal proteobacteria, Hirschia baltica gen.nov., sp. nov" 40 : 443-451, 1990

6 Lee, K., "Robiginitomaculumantarcticum gen. nov., sp. nov., a member of the family Hyphomonadaceae,from Antarctic seawater" 57 : 2595-2559, 2007

7 Quan, Z.X, "Rhizobium daejeonense sp. nov. isolated from a cyanidetreatment bioreactor" 55 : 2543-2549, 2005

8 Abraham, W.R, "Phylogenyof Maricaulis Abraham et al. 1999 and proposal of Maricaulisvirginensis sp. nov., M. parjimensis sp. nov., M. washingtonensissp. nov. and M. salignorans sp" 52 : 2191-2201, 2002

9 Abraham, W.R., "Phylogeny and polyphasic taxonomy ofCaulobacter species. Proposal of Maricaulis gen. nov. withMaricaulis maris (Poindexter) comb. nov. as the type species,and emended description of the genera Brevundimonas andCaulobacter" 49 : 1053-1073, 1999

10 Felsenstein,J, "Phylogenies and the comparative method" 125 : 1-15, 1985

11 Swofford,D.L, "PAUP. Phylogenetic Analysis Using Parsimony,version 3.1.1. Illinois Natural History Survey" Champaign 1993

12 Strömpl, C, "Oceanicaulisalexandrii gen. nov., sp. nov., a novel stalked bacterium isolatedfrom a culture of the dinoflagellate Alexandrium tamarense(Lebour) Balech" 53 : 1901-1906, 2003

13 Gerhardt, P., "Methods for General and Molecular Bacteriology" AmericanSociety for Microbiology, Washingto 1994

14 Kumar, S, "MEGA3: Integrated softwarefor molecular evolutionary genetics analysis and sequencealignment" 5 : 150-163, 2004

15 Komagata, K, "Lipid and cell-wall analysis inbacterial systematics" 19 : 161-207, 1987

16 Sasser,M, "Identification of Bacteria by Gas Chromatographyof Cellular Fatty Acids" MIDI, Newark 1990

17 Weiner, R.M., "Hyphomonasadhaerens sp. nov., Hyphomonas johnsonii sp. nov. andHyphomonas rosenbergii sp. nov., marine budding and prosthecatebacteria" 50 : 459-469, 2000

18 Moore, R.L., "Genus HyphomonasPongratz 1957 nom. rev. emend., Hyphomonas polumorphaPongratz 1957 nom. rev. emend., and Hyphomonasneptunium (Leifson 1964) comb. nov. emend. (Hyphomicrobiumneptunium)" 34 : 71-73, 1984

19 Desper, R, "Fast and accurate phylogeny reconstructionalgorithms based on the minimum-evolutionprinciple" 9 : 687-705, 2002

20 Jukes, T.H, "Evolution of protein molecules in:Mammalian Protein Metabolism" Academic Press 21-132, 1969

21 Williams, K.P, "A robustspecies tree for the ‘Alphaproteobacteria’" 189 : 4578-4586, 2007

22 Xu, H.X., "A rapid method for determining theG+C content of bacterial chromosomes by monitoring fluorescenceintensity during DNA denaturation in a capillarytube" 50 : 1463-1469, 2000

23 Reasoner, D.J, "A new medium for theenumeration and subculture of bacteria from potable water" 49 : 1-7, 1985

24 Gonzalez, J.M, "A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature" 4 : 770-773, 2002