배의 메탄올 추출물이 마우스의 비장세포 증식능과 Cytokine 생성능에 미치는 영향 = Effects of Pear(Pyrus pyrifolia) Methanol Extracts on the Proliferation and the Cytokines Production of Mouse Splenocytes

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the potential of pear (Pyrus pyrifolia) as a immune-modulating functional food by assay of splenocytes proliferation and induction of cytokines (IFN-γ, IL-4) in vitro. When mouse splenocytes were exposed to various concentration (0.16, 0.31, 0.63, 1.25, 2.50 ㎎/㎖) of pear methanol extracts (P-M) without mitogens, splenocytes proliferation (SP) was significantly increased. Also, SP to mitogens, concanavalin A (Con A) and lipopolysaccharide (LPS) were significantly increased by P-M when compared with controls. When splenocytes were cultured with P-M in the presence of Con A, cytokine (IFN-γ, IL-4) levels in culture supernatant were significantly enhanced in a dose-dependent manner except 2.5 ㎎/㎖ when compared with control group. Therefore, our study suggest that the pear has the potential of being an immune-modulating functional food.

참고문헌 (Reference)

1 "우리 배 이야기" 농촌진흥청 원예연구소 18-, 2003

2 "상황버섯 추출물이 정상마우스와 cyclophosphamide로 처리된 마우스의 체액성 면역기능에 미치는 영향" 9 : 194-, 2001

3 "function and role of lymphokines in regulating their development" Dutton tonko phe immu (tonko phe immu): 1991

4 "The effects of pear pectin on blood pressure ANP and cardiac hypertrophy in hypertensive rat induced by 2K1C" 32 : 2003

5 "The effects of fruit juices and fruits on the absorption of iron from a rice meal" 1987

6 "Sources of polycyclic aromatic hydrocarbon exposure in non-occupationally exposed koreans" 42 : 2003

7 "Selected Methods in Cellular Immunology" 14 : 1980

8 "Selected Methods in Cellular Immunology" 156 : 1980

9 "Rapid colorimetric assay for cellular growth and survival Application to proliferation and cytotoxicity assays" immu (immu): 1983

10 "In vitro evaluation of cell/biomaterial interaction by MTT assay" 1993

11 "Immunomodulatory properties of milk" 84 : 2000

12 "Immunologic effects of yogurt" 71 : 861-, 2000

13 "Heterogeneity of helper/inducer T lymphocytes" 1987

14 "Effects of polyphenol compound from korean pear on lipid metabolism" 33 : 2004

15 "Effects of pear phenolic compound on the STZ-treated mice for induction of diabetes" 31 : 1107-, 2002

16 "Effect of polyphenol compounds from korean pear on immunofunctional activity" 18 (18): 2003

17 "Effect of gastric myoelectric activity on carbohydrate absorption of fruit juice in children" 30 : 162-, 2000

18 "Antioxidant and antiproliferative activity of common fruits" 50 : 2002