Chitosan sponges as tissue engineering scaffolds for bone formation

다국어 초록 (Multilingual Abstract)

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 ㎛ pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4×10^(6)(day 1), 11×10^(6)(day 28) and 12×10^(6)(day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) ㎍ per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitotsan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and methods
• Materials
• Fabrication of chitosan sponge
• Cell seeding into the sponges and culture in vitro
• Osteoblastic cell proliferation
• Measurement of alkaline phosphatase activity
• Matrix mineralization analysis
• Histological examination of cell-sponge constructs in vitro culture
• Statistical analysis
• Results and discussion
• Chitosan sponge fabrication
• Osteoblast proliferation in the sponges
• Osteoblastic differentiation
• Histological observation
• Conclusions
• Acknowledgement
• References