This study was carried out to investigate the genetic polymorphisms of red cell protein, Haemoglobin(Hb) and serum protein, Post Transferrin-2 (PTf-2) through polyacrylamide gel electrophoresis(PAGE) in native Hanwoo and dairy Holstein breed.
Blood ...
This study was carried out to investigate the genetic polymorphisms of red cell protein, Haemoglobin(Hb) and serum protein, Post Transferrin-2 (PTf-2) through polyacrylamide gel electrophoresis(PAGE) in native Hanwoo and dairy Holstein breed.
Blood samples were collected for examination from a total of 180 cattle composed of 90 Hanwoo and 90 Holstein raising in Yesan area in Chungnam province.
The results obtained from this experiment were as follows;
1. The distributions of genotype for Hb loci in native Hanwoo were 62.9% for AA type, 17.1% for AB type, 10.0% for AC type, and 8.6% for CC type, respectively. However, homotype BC was not detected at these loci in the present study. And then, the whole distributions for Hb loci in dairy Holstein were only homotype AA.
2. The gene frequencies for HbA, HbB, and HbC in Hanwoo were 0.764, 0.099, and 0.150, respectively. However, in dairy Holstein only HbA gene was found in this study.
3. The distributions of genotype for PTf-2 loci in native Hanwoo were 53.3% for FF type, 36.6% for FS type, and 10.0% for SS type, respectively.
4. The gene frequencies for PTf-2F and PTf-2S in Hanwoo were 0.716 and 0.284, respectively.
5. According to above these results, the red cell, Hb loci in Hanwoo were supposed to be controlled by three autosomal codominant alleles, HbA, HbB, and HbC. Serum protein, PTf-2 loci were assumed to be controlled by two alleles.