Peptide-conjugated Gold Nanoparticle for the Norovirus Detection by Lateral Flow Assay

다국어 초록 (Multilingual Abstract)

Currently, virus detection is the most rapidly emerging market in the field of in vitro diagnostics. Norovirus, foodborne illnesses all over the world, is an infectious virus that causes acute gastroenteritis and is accompanied by various symptoms. Infection through food is not visible, so in vitro diagnosis is considered very necessary. Point-of-care testing is the effective and rapid diagnosis of a particular molecule in vitro. Several studies have been conducted as Lateral flow assay (LFA) fits the WHO’s standard of ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, deliverable) criteria among paper-based in vitro diagnostics (lateral flow assays, dipsticks, microfluidic paper-based analytical devices; μPAD, etc.). Traditional LFAs typically rely on antibody-based immunization, which requires a lot of cost and multiple processes due to the preprocessing process. Thus, peptides obtained from the evolutionary phase display can be a great alternative to antibodies. In this study, results were confirmed in LFA by synthesizing peptides binding norovirus and gold nanoparticles that are easy to observe in visible color. We have confirmed that visual detection is possible in very small amounts, 107 particles/ml. These results demonstrate the availability of peptides as alternatives to antibodies in existing LFA and effectively present economical and easy usability. Furthermore, simple changes in peptide sequences can be effectively detected for various targets.