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    창난 젓갈의 숙성 과정 중 미생물 및 자기소화효소 작용에 관한 연구 = Studies on Microbial and Enzymatic Actions during the Ripening Process of Salted Alaska Pollack Tripe

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    https://www.riss.kr/link?id=A104488003

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

    This study examined the roles of autolytic enzymes and microorganisms in the ripening process of salted Alaska pollack tripe made with various concentrations of salt i.e, 7.5% and 20% by weight. Salted Alaska pollack tripe treated with antibiotic agents for the inhibition of microbial growth and a control were prepared experimentally, and changes in chemical composition and viable cell counts were investigated, individually, during the ripening process. Just after the preparation of the low salt Alaska pollack tripe made with 7.5% salt, viable bacterial cells occurred at a level of 105 CFU/g. In the control, bacterial counts increased rapidly to 107 CFU/g by the 14th day of ripening. However, in the sample treated with antibiotic agents, counts were decreased to a level of 104 CFU/g by the 3rd day of ripening and increased gradually to 106 CFU/g by the 5th day of ripening, and then the same value was maintained there-after. Just after the preparation of the high salt Alaska pollack tripe made with 20% salt, viable bacterial cells occurred at a level of 103 CFU/g. In both the samples treated with antibiotic agents and the control, bacterial counts decreased rapidly to 100 CFU/g by the 45th day of ripening and increased gradually there-after. The content of amino type nitrogen was 76.3 ㎎% just after the preparation of the low salt Alaska pollack tripe made with 7.5% salt. Amino type nitrogen content was increased to 283.5 ㎎% by the 5th day of proper ripening in the control, but it was increased to 208.0 ㎎% in the sample treated with antibiotic agents.
    The difference in amino type nitrogen content was 75.5 ㎎/100 g. The content of amino type nitrogen was 57.2 ㎎% just after the preparation of the high salt Alaska pollack tripe made with 20% salt. Amino type nitrogen content was increased to 198.3 ㎎ by the 60th day of proper ripening in the control, but it was increased to 162.0 ㎎% in the sample treated with the antibiotic agents. The difference in amino type nitrogen content was 36.3 ㎎/100 g. The contents of VBN and TMA-N were 102.1 ㎎% and 20.5 ㎎%, respectively, at the 7th day of ripening in the low salt Alaska pollack tripe made with 7.5% salt. The content of VBN was 60.0 ㎎% and TMA-N was not detected at the 21st day of ripening in the sample treated with antibiotic agents. The control sample was spoiled by the 7th day of ripening but the sample treated with antibiotic agents was not spoiled by the 21st day of ripening. On the other hand, VBN content was 37.2 ㎎% and TMA-N was not detected at the 90th day of ripening in the high salt Alaska pollack tripe made with 20% salt, and the control sample was not spoiled.
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    This study examined the roles of autolytic enzymes and microorganisms in the ripening process of salted Alaska pollack tripe made with various concentrations of salt i.e, 7.5% and 20% by weight. Salted Alaska pollack tripe treated with antibiotic agen...

    This study examined the roles of autolytic enzymes and microorganisms in the ripening process of salted Alaska pollack tripe made with various concentrations of salt i.e, 7.5% and 20% by weight. Salted Alaska pollack tripe treated with antibiotic agents for the inhibition of microbial growth and a control were prepared experimentally, and changes in chemical composition and viable cell counts were investigated, individually, during the ripening process. Just after the preparation of the low salt Alaska pollack tripe made with 7.5% salt, viable bacterial cells occurred at a level of 105 CFU/g. In the control, bacterial counts increased rapidly to 107 CFU/g by the 14th day of ripening. However, in the sample treated with antibiotic agents, counts were decreased to a level of 104 CFU/g by the 3rd day of ripening and increased gradually to 106 CFU/g by the 5th day of ripening, and then the same value was maintained there-after. Just after the preparation of the high salt Alaska pollack tripe made with 20% salt, viable bacterial cells occurred at a level of 103 CFU/g. In both the samples treated with antibiotic agents and the control, bacterial counts decreased rapidly to 100 CFU/g by the 45th day of ripening and increased gradually there-after. The content of amino type nitrogen was 76.3 ㎎% just after the preparation of the low salt Alaska pollack tripe made with 7.5% salt. Amino type nitrogen content was increased to 283.5 ㎎% by the 5th day of proper ripening in the control, but it was increased to 208.0 ㎎% in the sample treated with antibiotic agents.
    The difference in amino type nitrogen content was 75.5 ㎎/100 g. The content of amino type nitrogen was 57.2 ㎎% just after the preparation of the high salt Alaska pollack tripe made with 20% salt. Amino type nitrogen content was increased to 198.3 ㎎ by the 60th day of proper ripening in the control, but it was increased to 162.0 ㎎% in the sample treated with the antibiotic agents. The difference in amino type nitrogen content was 36.3 ㎎/100 g. The contents of VBN and TMA-N were 102.1 ㎎% and 20.5 ㎎%, respectively, at the 7th day of ripening in the low salt Alaska pollack tripe made with 7.5% salt. The content of VBN was 60.0 ㎎% and TMA-N was not detected at the 21st day of ripening in the sample treated with antibiotic agents. The control sample was spoiled by the 7th day of ripening but the sample treated with antibiotic agents was not spoiled by the 21st day of ripening. On the other hand, VBN content was 37.2 ㎎% and TMA-N was not detected at the 90th day of ripening in the high salt Alaska pollack tripe made with 20% salt, and the control sample was not spoiled.

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    참고문헌 (Reference)

    1 조학래, "젓갈류의 유통기한 연장을 위한 연구" 한국식품과학회 34 (34): 652-660, 2002

    2 이나영, "감마선 조사기술을 이용하여 제조된 양념창란젓갈의 이화학적 품질특성" 한국식품과학회 35 (35): 1129-1134, 2003

    3 Kim DS, "The processing of seasoned and fermented oyster and its quality changes during the fermentation" 44 : 81-87, 2001

    4 Kim SM, "The effects of food additives on the shelf-life of low salted myungran-jeot" 25 : 937-943, 1996

    5 Mori K, "The aerobic bacteria in ripening process of Ika shiokara" 45 : 771-779, 1979

    6 Cha YJ, "Studies on the processing of low salt fermented sea foods" 18 : 206-213, 1985

    7 Baek SH, "Studies on the physicochemical properties in procesing of accelerated low saltfermented anchovy by adding koji" 9 : 385-391, 1996

    8 Mori K, "Studies on the microorganisms in salted and ripened squid meat product(Ika shiokara)-Ⅰ. Yeasts in ripening process of Ika shiokara" 43 : 1425-1432, 1977

    9 Baek SH, "Studies on the microflora and enzyme activity in procesing of accelerated low saltfermented anchovy by adding koji" 9 : 392-397, 1996

    10 Uno T, "Studies on the marine fermented foods.-7. On the preservative effects of glycerine and xylose added to squid shiokara" 33 : 19-31, 1976

    1 조학래, "젓갈류의 유통기한 연장을 위한 연구" 한국식품과학회 34 (34): 652-660, 2002

    2 이나영, "감마선 조사기술을 이용하여 제조된 양념창란젓갈의 이화학적 품질특성" 한국식품과학회 35 (35): 1129-1134, 2003

    3 Kim DS, "The processing of seasoned and fermented oyster and its quality changes during the fermentation" 44 : 81-87, 2001

    4 Kim SM, "The effects of food additives on the shelf-life of low salted myungran-jeot" 25 : 937-943, 1996

    5 Mori K, "The aerobic bacteria in ripening process of Ika shiokara" 45 : 771-779, 1979

    6 Cha YJ, "Studies on the processing of low salt fermented sea foods" 18 : 206-213, 1985

    7 Baek SH, "Studies on the physicochemical properties in procesing of accelerated low saltfermented anchovy by adding koji" 9 : 385-391, 1996

    8 Mori K, "Studies on the microorganisms in salted and ripened squid meat product(Ika shiokara)-Ⅰ. Yeasts in ripening process of Ika shiokara" 43 : 1425-1432, 1977

    9 Baek SH, "Studies on the microflora and enzyme activity in procesing of accelerated low saltfermented anchovy by adding koji" 9 : 392-397, 1996

    10 Uno T, "Studies on the marine fermented foods.-7. On the preservative effects of glycerine and xylose added to squid shiokara" 33 : 19-31, 1976

    11 Uno T, "Studies on the marine fermented foods.-6. On the preservative effects of voltile fatty acid monoglycerides added to squid shiokara" 32 : 31-39, 1975

    12 Uno T, "Studies on the marine fermented foods.-5. On the preservative effects of lactic acid added to squid shiokara" 31 : 23-33, 1974

    13 Uno T, "Studies on the marine fermented foods.-3. On the preservative effects of maltitol added to squid shiokara" 31 : 22-30, 1974

    14 Uno T, "Studies on the marine fermented foods.-1. On the flavor and preservative effects ofalcohol added to squid shiokara" 29 : 23-29, 1972

    15 Uno T, "Studies on the marine fermented foods-2. On the preservative effects of the sugars and monoglycerides added to squid shiokara" 30 : 23-32, 1973

    16 Kashiwada K, "Studies on the enzyme of skipjack, Katsuwonus vagans, entrails-Ⅰ. On the seasonal variation of proteolytic enzyme activity in pyloric coecca" 18 : 151-154, 1952

    17 Mori K, "Shiokara(salt-fermented fish product), The ripening process of food(Edit. by Sato N.)" Korin Ltd. Tokyo 631-653, 1984

    18 Lee WD, "Recent development of jeotkal(traditional Korean fermented seafood) and its future" 6 : 23-27, 2001

    19 Lee KH, "Production of low salted and fermented shrimp by irradiation" 67 : 1772-1777, 2000

    20 Kim YM, "Processing conditions of low salt fermented squid and its flavor components-2. Effect of temperature, salinity and pH on the growths of bacteria from isolated low salt fermented squid" 24 : 631-636, 1995

    21 KFRI, "Modernization Strategy for Traditional Food. Traditional Food Laboratory"

    22 Lee KH, "Microbiological and enzymological studies on the flavor components of sea food pickles" 11 : 1-27, 1968

    23 Collins CH, "Microbiological Methods" London Butterworths 127-, 1964

    24 Lee CH, "Korean Salt Fermented Sea Foods" Yulim publishing Co 14-, 1987

    25 Mori K, "Histological changes Ika shiokara during the ripening process" 46 : 1287-1292, 1980

    26 Park SH, "Hangeul SPSS" SPSS Academy Press 2005

    27 KFIA, "Food Code. Korea Food Industry Association"

    28 Van Veen AG, "Fermented and dried sea-food products in South-East Asia. In Fish as Food (Edit. by Borgstrom G.)" Academic Press, New York 227-250, 1965

    29 Beddows CG, "Fermented Fish and Fish Products(Edit. by Wood BJB), Microbiology of Fermented Foods" Elsevier Applied Science Publishers, London 1-39, 1985

    30 MMAF, "Development of new manufacturing process for jeotkal"

    31 Lee WD, "Development of new manufacturing process for Changranjeotgal- 1. Optimization of salting process" 34 : 114-118, 2001

    32 Lee WD, "Development of new manufacturing process for Changran-jeotgal-3. Improvement of seasoning process and quality estimation" 34 : 119-124, 2001

    33 Lee WD, "Development of new manufacturing process for Changran-jeotgal-2. Optimization of fermentation process" 34 : 109-113, 2001

    34 Cha YJ, "Characterization of the strong proteolytic bacteria isolated from low salt fermented anchovy and the protease produced by that strain" 21 : 71-79, 1988

    35 Lee CH, "Characteristics of Korean fish fermentation technology" 1 : 267-278, 1986

    36 Oh SC, "Changes of the volatile basic nitrogen and free amino acid according to the fermentation of low salt fermented squid" 16 : 173-181, 2000

    37 Jo C, "Application of gamma irradiation on manufacturing changran jeotkal (aged and seasoned intestine of Alaska pollack), Microbiological and sensory characteristics" 23 : 673-678, 2003

    38 Murray CK, "An investigation of the method of determining trimethylamine in fish muscle extracts by the formation of its picrate salt" 7 : 35-47, 1972

    39 Yoon JH, "A study in packing of changran-jeotkal-1. Shelf-life of a jar packing in changran-jeotkal" 35 : 8-14, 2002

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