Classification and Chemometric Study of Southern Italy Monovarietal Wines Based on NMR and HPLC-DAD-MS

다국어 초록 (Multilingual Abstract)

There is increasing interest in development of methods for wine characterization to guarantee traceability and product safety. Metabolomics represents a valuable tool to trace the metabolic fingerprint of complex mixtures.
Wines from 22 pure varieties (autochthonous from Southern Italy and grown in the same area of Apulia) were genetically identified and metabolically characterized using both NMR and HPLC-MS. NMR associated with chemometrics was used to identify amino acid (proline, alanine, and tyrosine), organic acid (malic, lactic, acetic, and succinic acids), alcohol (2-phenylethanol), sugar (sucrose), and polyphenol (gallic acid) contents. Minor components of wines were determined using HPLC-MS analyses. Wines showed potential health-promoting effects with good amounts of phenolic compounds. In particular, consistent levels of trans-resveratrol and flavonoids were found in Negro Amaro, Uva di Troia, Ancellotta, and Montepulciano wines.

참고문헌 (Reference)

1 Duarte J, "Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships" 24 : 857-862, 1993

2 Fischer U, "The impact of geographic origin, vintage and wine state on sensory properties of Vitis vinifera cv. Riesling wines" 10 : 281-288, 1999

3 Ogrinc N, "The application of NMR and MS methods for detection of adulteration of wine, fruit juices, and olive oil. A review" 376 : 424-430, 2003

4 Di Majo D, "The antioxidant capacity of red wine in relationship with its polyphenolic constituents" 111 : 45-49, 2008

5 Godelmann R, "Targeted and non-targeted wine analysis by 1H NMR spectroscopy combined with multivariate statistical analysis. Differentiation of important parameters: Grape variety, geographical origin, year of vintage" 61 : 5610-5619, 2013

6 Gasparro M, "Sangiovese and its offspring in southern Italy" 54 : 581-589, 2013

7 Reid LM, "Recent technological advances for the determination of food authenticity" 17 : 344-353, 2006

8 Cordella C, "Recent developments in food characterization and adulteration detection: Technique-oriented perspectives" 50 : 1751-1764, 2002

9 Crupi P, "Post-harvest stilbenes and flavonoids enrichment of table grape cv Redglobe (Vitis vinifera L.) as affected by interactive UV-C exposure and storage conditions" 141 : 802-808, 2013

10 De Nisco M, "Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (cv. Aglianico)" 140 : 623-629, 2013

11 Bakker J, "Model wine solutions: Colour and composition changes during aging" 32 : 111-118, 1993

12 Fiehn O, "Metabolomics-the link between genotypes and phenotypes" 48 : 155-171, 2002

13 Son HS, "Metabolomic studies on geographical grapes and their wines using 1H NMR analysis coupled with multivariate statistics" 57 : 1481-1490, 2009

14 Ali K, "Metabolic characterization of Palatinate German white wines according to sensory attributes, varieties, and vintages using NMR spectroscopy and multivariate data analyzes" 49 : 255-266, 2011

15 Dettmer K, "Mass spectrometry-based metabolomics" 26 : 51-78, 2007

16 Nilsson M, "High-resolution NMR and diffusion-ordered spectroscopy of port wine" 52 : 3736-3743, 2004

17 Ribereau-Gayon P, "Handbook of Enology: The Chemistry of Wine Stabilization and Treatments" Wiley 1-205, 2006

18 Crupi P, "HPLC-DAD-ESI-MS analysis of flavonoid compounds in five seedless table grapes grown in Apulian Region" 77 : C174-C181, 2012

19 Rodríguez-Bernaldo de Quirós A, "HPLC analysis of polyphenolic compounds in Spanish white wines and determination of their antioxidant activity by radical scavenging assay" 42 : 1018-1022, 2009

20 Vitrac X, "Direct liquid chromatographic analysis of resveratrol derivatives and flavanonols in wines with absorbance and fluorescence detection" 458 : 103-110, 2002

21 Brescia MA, "Characterization of the geographical origin of Italian red wines based on traditional and nuclear magnetic resonance spectrometric determinations" 458 : 177-186, 2002

22 Jang M, "Cancer chemopreventive activity of resveratrol, a natural product derived from grapes" 275 : 218-220, 1997

23 Galgano F, "Authentication of Italian red wines on the basis of the polyphenols and biogenic amines" 232 : 889-897, 2011

24 Belton PS, "Application of chemometrics to the 1H NMR spectra of apple juices: discrimination between apple varieties" 61 : 207-213, 1998

25 Sánchez-Moreno C, "Anthocyanin and proanthocyanidin content in selected white and red wines. Oxygen radical absorbance capacity comparison with nontraditional wines obtained from Highbush blueberry" 51 : 4889-4896, 2003

26 García-Beneytez E, "Analysis of grape and wine anthocyanins by HPLC-MS" 51 : 5622-5629, 2003

27 Castellari M, "An improved HPLC method for the analysis of organic acids, carbohydrates, and alcohols in grape musts and wines" 23 : 2047-2056, 2000

28 López-Rituerto E, "A thorough study on the use of quantitative 1H NMR in Rioja red wine fermentation process" 57 : 2112-2118, 2009

29 Pereira GE, "1H NMR metabolite fingerprints of grape berry: Comparison of vintage and soil effects in Bordeaux grapevine growing areas" 563 : 346-352, 2006