밤나무 잎 추출물의 항노화 활성 및 화장품에의 응용(Ⅰ) = Anti-oxidative Activities of Castanea crenata Leaf Extract/Fractions and Application on Cosmetics

다국어 초록 (Multilingual Abstract)

In this study, the antioxidative effects, inhibitory effects on tyrosinase and elastase and components of Castanea crenata leaf were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl radical, DPPH) scavenging activity (FSC₅₀) of extract / fractions of Castanea crenata leaf was in the order: 50 % ethanol extract (13.6 µg/mL) < ethyl acetate fraction (6.2) < aglycone fraction (2.1). Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of extract/fractions from Castanea crenata leaf extract / fractions on ROS generated in Fe³⁺-EDTA/H₂O₂ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity was in the order: aglycone fraction (0.8) < 50 % ethanol extract (0.5) < ethyl acetate fraction (0.3). The scavenging activity (IC₅₀) for O₂^·-(superoxide anion radical) generated by NBT method was in the order: ethyl acetate fraction (145.5) < aglycone fraction (65.5). The protective effects on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction exhibited the most prominent cellular protective effect (τ₅₀, 191.9 ± 12.2 min at 10 µg/mL). The inhibitory effect of aglycone fraction (9.1 µg/mL) on elastase was higher than oleanolic acid (13.7 µg/mL). And the inhibitory effect of aglycone fraction (21.6 µg/mL) on tyrosinase was higher than arbutin (226.2 µg/mL). But 50 % ethanol extract rarely exhibited the inhibitory activity on tyrosinase and elastase. Flavonoids were contained in Castanea crenata leaf (96.3 mg / 100 g dried Castanea crenata leaf). And flavonoids contained in ethyl acetate fraction were kaempferol, quercetin, quercitrin, and so on. Quercitrin is the most abundant component. These results indicate that extract / fractions of Castanea crenata can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging free radical and ROS, Castanea crenata leaf extract / fractions could be used as a new cosmeceutical for whitening and anti-wrinkle products.

참고문헌 (Reference)

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