죽황(竹黃)으로부터 분리한 미백활성 성분의 멜라닌생성 억제효과|RISS 상세보기

다국어 초록 (Multilingual Abstract)

In order to develop a new depigmenting agent, extracts were obtained from 60 native plants and their antimelanogenic activities were screened by evaluating the inhibitory effect on tyrosinase which is a major enzyme responsibles for the melanin synthesis. The extracts of Trichosanthes kirilowii fruits, Phyllostachys bambusoides inner films (BIF), Clerodendrum trichotomum leaves, and Acer okamotoanum leaves showed relatively high inhibitory effect on tyrosinase and their IC<sub>50</sub> values were 50 ∼ 100 μg/mL. The extract of BIF inhibited melanin synthesis of B16F10 melanoma cells by 52 %, which was the highest among those of various extracts. Furthermore, the effect of BIF extract is 10 % higher than that of arbutin (42 %), a popular depigmenting agent in Korea. Ten compounds having antimelanogenic activity were isolated from the BIF extract by solvent extraction and chromatography. These compounds were identified as phenolic derivatives: SM701, SM702, SM703, and BPR211 were hydroquinone derivatives; SM707 a gallic acid derivative; SM704, SM705, SM706, SM708 and SM709 ferulic acid derivatives. The free radical scavenging activities of these compounds were measured and compared to those of hydroquinone and vitamin C. The SC<sub>50</sub> values scavenging 50 % DPPH of SM702 and SM709 were 60 ∼ 70 μM similar to that of hydroquinone and those of SM701 and SM708 were 30 ∼ 40 μM slightly lower than that of vitamin C. These results suggest the presence of components having high antioxidant activity in the BIF extract. The SM709, identified as 1,2-O-diferulylglycerol, inhibited the activities of tyrosine hydroxylase and dopa oxidase by 18 and 60 %, respectively. The SM709 also inhibited the melanin synthesis of B16F10 melanoma cells by 62 % and this was the highest antimelanogenic activity among those obtained from the various purified compounds. Therefore, antimelanogenic activity of the BIF extract was concluded to be due to both inhibition of DOPA oxidase and antioxidant activity.

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참고문헌 (Reference)

1 J. H. Park, "Tyrosinase inhibition activity of some herbal drugs" 41 (41): 518-, 1997

2 S. H. Lee, "The screening of the inhibitory compounds on tyrosinase activity from the natural product" 41 (41): 456-, 1997

3 F. Nanjo, "Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical" 21 (21): 895-, 1996

4 T. Mosmann, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays" 65 (65): 55-, 1983

5 H. Ando, "Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis" 40 (40): 1312-, 1999

6 S. Tuomaala, "Population-based assessment of clinical characteristics predicting outcome of conjunctival melanoma in whites" 43 (43): 3399-, 2002

7 A. G. Lowell, "Physiology, biochemistry and molecular biology of the skin" Oxford University Press Inc. 891-, 1991

8 H. Shimomura, "Phenolic glycerides from Lilium auratum" 26 (26): 844-, 1987

9 H. Shimomura, "New phenylpropanoid glycerol glucosides from the bulbs of Lilium species" 36 (36): 4841-, 1988

10 D. I. Jang, "Melanogenesis inhibitor from paper mulberry" 112 (112): 59-, 1997