High Prevalence of AmpC β-Lactamases in Clinical Isolates of Escherichia coli in Ilam, Iran

다국어 초록 (Multilingual Abstract)

Objectives: Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aimof this study is to determine themolecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran. Methods: One hundred and twelve clinical isolates of E. coli were collected from hospitalized patients and were identified by biochemical tests. They were evaluated for extended spectrum beta-lactamases (ESBLs) production, and the positive strains were subjected to AmpC enzymes; for detection of AmpC cluster genes, multiplex polymerase chain reaction was applied. Results: The analysis showed 62.5% of isolates were ESBLs positive and that five strains revealed the AmpC cluster genes. This is the first report of FOXM cluster genes in E. coli in Iran. Conclusion: Based on our results, the prevalence of AmpC β-lactamases is increasing in Iran, which caused failure in antibiotic therapy. So, the current study recommended the revision of antibiotic policy in Iranian hospitals.

참고문헌 (Reference)

1 Babic M, "What’s new in antibiotic resistance? Focus on beta-lactamases" 9 (9): 142-156, 2006

2 Ding H, "The prevalence of plasmid-mediated AmpC β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae from five children’s hospitals in China" 27 (27): 915-921, 2008

3 Paterson DL, "Resistance in gram-negative bacteria: enterobacteriaceae" 1119 (1119): S20-S28, 2006

4 Dallai MS, "Prevalence of AmpC and SHV β-lactamases in clinical isolates of Escherichia coli from Tehran hospitals" 6 (6): 176-180, 2013

5 Phillppon A, "Plasmid-determined AmpC-type beta-lactamases" 46 (46): 1-11, 2002

6 Bredford P, "Extended-spectrum ß-lactamases in the 21st century:characterization, epidemiology and detection of this important resistance threat" 933-951, 2001

7 Mansouri S, "Determination of resistance pattern of plasmid-mediated ampc β-lactamases producing isolate of Escherichia coli" 16 (16): 60-71, 2009

8 Sanders C, "Chromosomal cephalosporinases responsible for multiple resistance to newer b-lactam antibiotics" 41 (41): 573-594, 1987

9 Alireza Japoni-Nejad, "Characterization of Plasmid-Mediated AmpC and Carbapenemases among Iranain Nosocomial Isolates of Klebsiella pneumoniae Using Phenotyping and Genotyping Methods" 질병관리본부 5 (5): 333-338, 2014

10 Poirel L, "Carbapenemases: molecular diversity and clinical consequences" 2 (2): 501-512, 2007

11 Reller LB, "Antimicrobial susceptibility testing: a review of general principles and contemporary practices" 49 (49): 1749-1755, 2009

12 Fakioglu E, "Amp C β-lactamase-producing Escherichia coli in neonatal meningitis: diagnostic and therapeutic challenge" 26 (26): 515-517, 2006