FRET 기반 고분자 센서의 DNA 검출 감도 향상을 위한 연구 = Controlled FRET to improve detection sensitivity in conjugated polymer-based DNA biosensor

다국어 초록 (Multilingual Abstract)

Signal amplification in fluorescence resonance energy transfer (FRET)-based DNA detection was investigated by using cationic polyfluorene as a FRET donor and fluorescein (Fl)-labeled single stranded DNA (ssDNA-Fl) as a FRET acceptor for improving detection sensitivity. The FRET rate is very sensitive to the intermolecular distance and the FRET efficiency increases with decreasing distance between FRET donor (D) and acceptor (A). However, the energy wasting photo-induced charge transfer (PCT) quenching also increases with decreasing D-A intermolecular distance. The competition between the FRET and PCT processes can be controlled by modifying D-A intermolecular distance. The Layer-by-Layer (LbL) assembling technique is expected to provide a fine-control of the FRET D-A intermolecular distance. By LbL assemblies, we controlled the number of inter layers between cationic polyfluorene and ssDNA-Fl, and the resulting FRET-induced signal was successfully modulated by changing the intermolecular D-A distance on the molecular level. Therefore, the LbL technique can suggest an efficient way to fine-tune the competition between FRET and PCT for maximizing detection sensitivity in FRET-based DNA biosensors.

참고문헌 (Reference)

1 A. Moliton, 53 : 1397-, 2004

2 Gotz, M, 41 : 4156-, 2002

3 J. Kim, 39 : 3868-, 2008

4 B. Liu, 34 : 7932-, 2001

5 A. Rose, 434 : 876-, 2005

6 Woo, H. Y, 17 : 290-, 2007

7 Q. Zhou, 117 : 12593-, 1995

8 H. A. Ho, 41 : 1548-, 2002

9 S. Tyagi, 14 : 303-, 1996

10 R. H. Friend, 397 : 121-, 1999

11 Gaylord, B. S, 99 : 10954-, 2002

12 Heeger, P. S, 96 : 12219-, 1999

13 Xu, Q.-H, 101 : 11634-, 2004

14 Kang, M, 42 : 2708-, 2009

15 Nag, O. K, 113 : 5788-, 2009

16 Ray, K, 19 : 5902-, 2007

17 Liu, B, 128 : 1188-, 2006

18 Lakowicz, J. R, "Principles of Fluorescence Spectroscopy" Kluwer Academic/Plenum 1999