산수유 에탄올 추출물의 Sarcoma-180 세포에 대한 항암 효과

국문 초록 (Abstract)

산수유를 항암 식품 소재로 활용하기 위하여 sarcoma-180 암세포에 대한 산수유 에탄올 추출물의 증식 억제 및 마우스에서의 수명 연장효과를 검토하였다. 산수유 에탄올 추출물을 10, 100, 300 및 500 ㎍/㎖ 농도로 24, 48 및 72시간 별로 sarcoma-180 암세포에 처리하여 암세포 증식억제 효과를 측정한 결과, 농도 및 시간 의존적으로 억제하였으며, 500 ㎍/㎖ 농도에서 48시간 배양 시 60% 이상의 높은 암세포 증식 억제 효과를 나타내었다. 산수유 에탄올 추출물을 100 ㎍/㎖ 농도 이상으로 처리한 암세포에서는 대조군의 증식이 감소되었으며, 형태학적 변화도 관찰되었다. 또한 산수유 에탄올 추출물은 농도 의존적으로 핵을 응축하고 apoptotic bodies를 생성하였으며, 300 및 500 ㎍/㎖의 농도 에서는 DNA fragmentation을 유도하였고 이러한 apoptosis 유도는 caspase-3 활성과 관련이 있었다. 산수유 에탄올 추출물을 sarcoma-180 암세포가 접종된 마우스 복강에 7일간 100 및 300 ㎎/㎏/day의 농도로 연속적으로 투여하였을 때, 산수유 투여군의 수명이 대조군에 비하여 증가되었다. 따라서 이 결과를 종합하여 볼 때 산수유는 암 예방 기능성 소재로서 활용이 가능하리라 생각된다.

다국어 초록 (Multilingual Abstract)

To develop Corni Fructus as a cancer preventive food material, the in vitro cytotoxicities and in vivo antitumor activities of various concentrations of 80% Corni Fructus ethanol extract (CFEE) were investigated using sarcoma-180 cancer cell. Viability was decreased and cell death rate was increased in both dose- and time-dependent manners in cells treated with CFEE at 10, 100, 300, and 500 ㎍/㎖ concentrations for 24, 48, and 72 hr. Proliferation was also inhibited more than 60% in cells treated with CFEE at the 100 ㎍/㎖ concentration for 48 hr. In addition, the morphology of cells treated with CFEE at the 100 and 500 ㎍/㎖ concentrations was distorted with shrunken cell masses and lower cell numbers compared to the control cells. In the cells treated with CFEE, the formation of apoptotic bodies and nuclear condensation were observed in dose dependent manners. CFEE also increased DNA fragmentation values at the 100 and 500 ㎍/㎖ concentrations. The apoptosis induced by CFEE was connected to the proteolytic activation of caspase-3. When CFEE was administered at 100 and 300 ㎎/㎏, ip, for 7 consecutive days in mice inoculated with sarcoma-180 cancer cell, the life span of the mice was found to be longer than that of the control mice that did not receive the extract. These results suggest that Corni Fructus may be used as a potential cancer preventive food material.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 문헌

참고문헌 (Reference)

1 채희정, "항암소재의 발굴을 위한 한방소재의 선별" 한국생물공학회 22 (22): 139-145, 2007

2 권승혁, "인체 전립선암세포에서 산수유 에탄올 추출물의 환경호르몬에 대한 방어효과" 한국식품영양과학회 38 (38): 663-666, 2009

3 김용두, "산수유의 용매분획별 항균활성" 한국식품영양과학회 32 (32): 829-832, 2003

4 김병현, "산수유에 함유된 항암물질의 정제 및 특성" 한국식품과학회 36 (36): 1001-1007, 2004

5 박찬성, "산수유, 황기, 감초 추출물의 생리활성" 대한본초학회 23 (23): 93-101, 2008

6 전연희, "산수유(Cornus officianalis) 에탄올 추출물의 항산화, 항돌연변이 활성 및 암세포 성장 억제 효과" 한국식품영양과학회 37 (37): 1-7, 2008

7 김옥경, "산수유 추출물이 Streptozotocin으로 유발된 흰쥐의 항당뇨 및 항산화 작용에 미치는 효과" 한국유화학회 22 (22): 157-167, 2005

8 한성희, "산수유 추출물의 납 제거 효과에 미치는 영향" 한국식생활문화학회 18 (18): 544-550, 2003

9 Zuckerberg C, "Ultrastructure of sarcoma 180" 10 : 272-282, 1973

10 Chung SR, "Toxicity and lectins constituents from the seed of Cornus officinalis" 24 : 177-182, 1993

11 Meng YQ, "The synthesis of ursolic acid derivatives with cytotoxic activity and the investigation of their preliminary mechanism of action" 17 : 848-854, 2009

12 Kuo PL, "The mechanism of ellipticine-induced apoptosis and cell cycle arrest in human breast MCF-7 cancer cells" 223 : 293-301, 2005

13 Sur SW, "The experimental study of proliferating activity of transplanted sarcoma 180 into Gingiva" Kyung Hee University 1992

14 Park YK, "The antidiabetic effects of extracts from Cornus officinalis seed" 9 : 5-11, 1995

15 서영배, "Study on the Effects of Corni Fructus about the Anti-allergic Action" 대한본초학회 17 (17): 1-12, 2002

16 Won DH, "Studies on the analysis of Corni fructus and its preparation" 1 : 197-201, 1996

17 Fukushima M, "Studies on cosmetic ingredients from crude drugs (Ⅰ)" 43 : 142-147, 1989

18 Guilian T, "Separation of gallic acid from Cornus officinalis Sieb. et Zucc by high-speed counter-current chromatography" 886 : 309-312, 2000

19 Zhang YX, "Phosphorylation of Bcl-2 and activation of caspase-3 via the c-Jun N-terminal kinase pathway in ursolic acid-induced DU145 cells apoptosis" 91 : 1173-1179, 2009

20 채명종, "Peroxisome proliferator-activated receptorγ 배위자의 간암 세포에 대한 항암 효과" 대한내과학회 75 (75): 288-299, 2008

21 Lee JY, "Iridoid glycosides of Cornus officinalis" Seoul National University 1981

22 Chan FL, "Induction of apoptosis in prostate cancer cell lines by a fla-vonoid, baicalin" 160 : 219-228, 2000

23 Toheu E, "Constituents of Cornus officinalis" 93 : 30-36, 1973

24 Xu W, "Arrested proliferation and molecular mechanism of MAPKs' activations in manganese-treated PC12 cell line" 33 : 674-, 2004

25 Kim YE, "Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts" 2 : 141-144, 1997

26 Seo KI, "Antimicrobial and antioxidative activities of Corni fructus extracts" 6 : 99-103, 1999

27 Korea National Statistical Office, "Annual report on the cause of death statistics" 2008

28 Jeng H, "A substance isolated from Cornus officinalis enhances the motility of human sperm" 25 : 301-306, 1997