동해 울릉도-독도해역에서 질소, 인, 철 첨가실험에 따른 식물플랑크톤의 성장 및 군집반응

국문 초록 (Abstract)

동해 울릉도-독도 2정점에서 식물플랑크톤의 군집조성 및 수직적 영양염분포 특성과 표층 현장수를 이용하여 N, P, NP, Fe을 첨가하여 식물플랑크톤의 영양염소비 및 성장특성을 파악하였다. 현장에서 영양염류농도는 유광층 상부에서 낮았고, 100 m보다 깊은 수심에서 증가하였다. N:P비는 Redfield비(16)보다 표층에서 21-25로 높았고, 조사 최대심도인 400 m층에서는 6 전후로 극히 낮게 관찰되었다. 식물플랑크톤의 총개체수는 St.UD3과 St.50에서 각각 4.9 X 105 cells L-1과 1.9 X 105 cells L-1로 관찰되었다. 주요 우점종은 St.UD3에서 침편모조류 Heterosigma akashiwo, 은편모조류 Crytomonas spp., 규조류 Leptocylindrus danicus로 파악되었고, St.50에서는 규조류 Chaetoceros socialis, H. akashiwo, L. danicus로 각각 관찰되었다. 영양염첨가실험의 +N과 +NP실험군에서 빠른 식물플랑크톤의 성장반응을 보였고, +P실험군에서는 대조 군과 유사하게 식물플랑크톤 성장이 관찰되지 않았다. 아울러 Fe을 첨가한 대조군, +N, +P, +NP실험군에서 Fe을 첨가하지 않은 다른 실험군과 비교하여 형광값의 유의한 차이를 보이지 않았다는 것은 Fe첨가에 따른 식물플랑크톤의 성장반응이 명확하지 않다는 것을 의미한다(p>0.05). 결과적으로 현장에서 우점한 H. akashiwo 와 L. danicus는 영양염이 추가적으로 공급된 환경의 생물검증실험에서도 빠르게 반응하여 높은 개체수로 우점한 것을 파악하였고, 특히 +Fe 실험군에서 L. danicus의 빠른 성장이 두드려졌다.

다국어 초록 (Multilingual Abstract)

We investigated phytoplankton and vertical inorganic nutrients at two stations around water of Ulleungdo and Dokdo in the East Sea, Korea. Nutrient addition experiments (+N, +P, +NP and +Fe) were also conducted to determine the growth response and nutrient consumption of the phytoplankton assemblage using the surface water of St. UD3 and St.50. In the field, although inorganic nutrients were low in the euphotic layer, these nutrients were increased at depths below 100 m. The total phytoplankton abundances in St. UD3 and St.50 were 4.9 X 105 cells L-1 and 1.9 X 105 cells L-1, respectively. The dominant species at St. UD3 was observed to be Raphidophyta Heterosigma akashiwo, Cryptophyta Crytomonas spp., and diatom Leptocylindrus danicus, while L. danicus and H. akashiwo including small diatom species Chaetoceros socialis were dominant at St. 50. In the nutrient addition experiments, phytoplankton growth (in vivo flourescence) in the +N and +NP treatment was 2-3 times higher than that in the +P treatment, indicating that the natural phytoplankton can respond to pulsed nutrient loading events. In addition, in vivo fluorescence in +Fe treatment was not statistically (p>0.05) different from that of the non-Fe treatments, indicating that the phytoplankton growth response in +Fe treatment was not significant. Dominant H akashiwo and L. danicus in the field showed a rapid response in nutrient additional bio-algal assay, particularly L. danicus in the +Fe treatments.

목차 (Table of Contents)

• 요약
• Abstract
• 1. 서론
• 2. 재료 및 방법
• 3. 결과 및 고찰
• 4. 결론
• References

참고문헌 (Reference)

1 이동섭, "동해 해수의 질소:인의 비" 한국해양학회 20 (20): 199-205, 2015

2 최동한, "독도 해역 초미소남세균 다양성의 계절적 변동" 한국해양학회 20 (20): 192-198, 2015

3 H, Yamaguchi, "The utilization of organic phosphorus by eukaryotic phytoplankton in marine environments (Review)" 57 : 1-12, 2010

4 A. C. Redfield, "The biological control of chemical factors in environment" 46 : 205-221, 1958

5 Seung Ho Baek, "Strong bottom–up effects on phytoplankton community caused by a rainfall during spring and summer in Sagami Bay, Japan" Elsevier BV 75 (75): 253-264, 2009

6 DM Nelson, "Silicic acid depletion and silicon limitation in the plume of the Mississippi River:evidence from kinetic studies in spring and summer" Inter-Research Science Center 136 : 163-178, 1996

7 Seung Ho Baek, "Seasonal distribution of phytoplankton assemblages and nutrient-enriched bioassays as indicators of nutrient limitation of phytoplankton growth in Gwangyang Bay, Korea" Elsevier BV 163 : 265-278, 2015

8 Hae Jin Jeong, "Red tides in Masan Bay, Korea in 2004–2005: I. Daily variations in the abundance of red-tide organisms and environmental factors" Elsevier BV 30 : S75-S88, 2013

9 A. R. Dzialowski, "Nutrient limitation of phytoplankton growth in central plain reservoirs, USA" 27 : 587-595, 2005

10 Thomas H. Chrzanowski, "Effects of mineral nutrients on the growth of bacterioߚ and phytoplankton in two southern reservoirs" Wiley-Blackwell 46 (46): 1319-1330, 2001

11 C. Labry, "Competition for phosphorus between two dinoflagellates: A toxic Alexandrium minutum and a non-toxic Heterocapsa triquetra" Elsevier BV 358 (358): 124-135, 2008

12 K.-I. Chang, "Circulation and currents in the southwestern East/Japan Sea: Overview and review" Elsevier BV 61 (61): 105-156, 2004

13 Craig MacPhee, "BIOASSAY OF ALGAL PRODUCTION IN CHEMICALLY ALTERED WATERS" Wiley-Blackwell 6 (6): 416-422, 1961

14 T. R. Parsons, "A Manual of Chemical and Biological Methods for Seawater Analysis" Pergamon Press 173-, 1984