제주산 왁스애플 추출물의 항염 활성

다국어 초록 (Multilingual Abstract)

The present study aimed to investigate comparative anti-inflammatory effects of Syzygium samarangense (SS, Wax apple) leaf and branch extract considering the balance of safety and efficacy. SS is a plant species in the family Myrtaceae, native to an area that includes the Greater Sunda Islands, Malay Peninsula but now cultivated in Jeju Island. Dose response studies were conducted to determine the inhibitory effect of extracts on the expression of inflammatory enzymes: Prostaglandin (PGE₂) and nitric oxide (NO) inflammatory cytokine production, TNF-α, interleukin (IL)-6, interleukin (IL)-1β and inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. As a result, SS were shown higher anti-inflammatory activity. SS inhibited production of PGE2, NO and mRNA expression of iNOS, COX-2 and decreased TNF-α, IL-6, IL-1β induced by LPS stimulation in RAW 264.7 cells. SS at 800 μg/mL showed inhibitory effects on almost all the inflammatory mediators we examined except for all study regulation. In conclusion, this study suggest that SS could be a potential source as a new natural anti-inflammatory agent.

목차 (Table of Contents)

• Abstract
• 1. INTRODUCTION
• 2. MATERIALS AND METHOD
• 3. RESULTS AND DISCUSSION
• 4. CONCLUSION
• REFERENCES

참고문헌 (Reference)

1 윤현정, "마우스 대식세포인 RAW 264.7 세포에서 茵陳蒿湯의 항염증 효과" 대한본초학회 23 (23): 169-178, 2008

2 곽충실, "달맞이순과 다래순 에탄올 추출물의 in vitro 항산화효과 및 항염증효과" 한국식품영양과학회 43 (43): 207-215, 2014

3 남궁승, "까마귀쪽나무 열매와 잎의 항염증 효과 비교 연구" 한국자원식물학회 28 (28): 145-152, 2015

4 Aktan, F, "iNOS-mediated nitric oxide production and its regulation" 75 : 639-653, 2004

5 Willeaume, V, "Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences" 46 : 1-12, 1995

6 Smith, W. L, "The enzymology of prostaglandin endoperoxide H synthases-1 and -2" 68 : 115-128, 2002

7 Zhang Y. J, "The effects of Syzygium samarangense, Passiflora edulis and Solanum muricatum on alcohol- induced liver injury" 17 : 1616-, 2016

8 Mitchell, J. A, "Selectivity of nonsteroidal antiinflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase" 90 : 11693-11697, 1993

9 Seibert, K, "Role of inducible cyclooxygenase (COX-2) in inflammation" 4 : 17-23, 1994

10 Masferrer, J. L, "Regulation of prostaglandin synthesis by glucocorticoids" 4 : 25-30, 1994

11 Park, J. C, "Pharmacological action of herbal medicine on tropical fruit, good for health and rich in nutritional ingredient (KOR)," PlunHangbok 2013

12 Tae Hoon Lee, "Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells" 한국분자세포생물학회 23 (23): 398-404, 2007

13 Vane, J. A, "Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like durgs" 23 : 232-235, 1971

14 Kaplanski, "IL-6: A regulator of the transition from neutrophil to monocyte recruitment during inflammation" 24 : 25-29, 2003

15 Funk, C. D, "Human platelet/erythroleukemia cell prostaglandin G/H synthase : cDNA cloning, expression, and gene chromosomal assignment. FASEB J" 5 : 2304-2312, 1991

16 Nishida, T, "Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-kB" 52 : 1890-1896, 2007

17 William E. S, "Early estimation of myocardial damage in conscious dogs and patients with evolving acute myocardial infarction" 52 : 2579-2590, 1973

18 Simirgiotis M. J, "Cytotoxic chalcones and antioxidants from the fruits of a Syzygium samarangense (Wax Jambu)" 107 : 813-819, 2008

19 Crofford, L. J, "COX-1 and COX-2 tissue expression: Implications and predictions" (Suppl. 49) : 15-19, 1997

20 Soubir, T, "Antioxidant activities of some local bangladeshi fruits (Artocarpus heterophyllus, Annona squamosa, Terminalia bellirica, Syzygium samarangense, Averrhoa carambola and Olea europa)" 23 : 257-261, 2007