Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

다국어 초록 (Multilingual Abstract)

Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

참고문헌 (Reference)

1 Joffret ML, "Whole genome sequencing of enteroviruses species A to D by high-throughput sequencing : application for viral mixtures" 9 : 2339-, 2018

2 Ramírez H, "Small ruminant lentiviruses : genetic variability, tropism and diagnosis" 5 (5): 1175-1207, 2013

3 Sun Y, "Serological investigation of caprine arthritis encephalitis and ovine progressive pneumoniina in 11 provinces of China" 48 (48): 34-38, 2018

4 Carrozza ML, "Seroconversion against SU5 derived synthetic peptides in sheep experimentally infected with different SRLV genotypes" 137 (137): 369-374, 2009

5 Hötzel I, "Sequence similarity between the envelope surface unit(SU)glycoproteins of primate and small ruminant lentiviruses" 69 (69): 47-54, 2000

6 Michiels R, "Phylogenetic analysis of Belgian small ruminant lentiviruses supports cross species virus transmission and identifies new subtype b5 strains" 9 (9): 183-, 2020

7 Shah C, "Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates : evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade" 319 (319): 12-26, 2004

8 Singh R, "Pathology and polymerase chain reaction detection of ovine progressive pneumonia(maedi)cases in slaughtered sheep in India" 10 (10): 1401-1406, 2017

9 Slatko BE, "Overview of next-generation sequencing technologies" 122 (122): e59-, 2018

10 Schuster SC, "Next-generation sequencing transforms today's biology" 5 (5): 16-18, 2008

11 Skraban R, "Naturally occurring mutations within 39 amino acids in the envelope glycoprotein of Maedi-Visna virus alter the neutralization phenotype" 73 (73): 8064-8072, 1999

12 Tanaka M, "Mutations of conserved residues in the major homology region arrest assembling HIV-1 gag as a membrane-targeted intermediate containing genomic RNA and cellular proteins" 90 (90): 1944-1963, 2015

13 Olech M, "Molecular characterization of small ruminant lentiviruses of subtype A5 detected in naturally infected but clinically healthy goats of Carpathian breed" 9 (9): 992-, 2020

14 Pépin M, "Maedi-Visna virus infection in sheep: a review" 29 (29): 341-367, 1998

15 Gomez-Lucia E, "Maedi-Visna virus : current perspectives" 9 : 11-21, 2018

16 Lu R, "Genomic characterisation and epidemiology of 2019 novel coronavirus : implications for virus origins and receptor binding" 395 (395): 565-574, 2020

17 Grego E, "Genetic heterogeneity of small ruminant lentiviruses involves immunodominant epitope of capsid antigen and affects sensitivity of single-strain-based immunoassay" 9 (9): 828-832, 2002

18 Muz D, "First molecular characterization of Visna/Maedi viruses from naturally infected sheep in Turkey" 158 (158): 559-570, 2013

19 Kalogianni AI, "Etiology, epizootiology and control of MaediVisna in dairy sheep: a review" 10 (10): E616-, 2020

20 Olech M, "Epidemiological survey in single-species flocks from Poland reveals expanded genetic and antigenic diversity of small ruminant lentiviruses" 13 (13): e0193892-, 2018

21 Li R, "De novo assembly of human genomes with massively parallel short read sequencing" 20 (20): 265-272, 2010

22 "Chapter 3.7.2 Caprine Arthritis/Encephalitis and Maedi-Visna" OIE

23 Simpson JT, "ABySS : a parallel assembler for short read sequence data" 19 (19): 1117-1123, 2009

24 Zhang KS, "A seroprevalence survey of Maedi-Visna among twenty-four ovine floks from twelve regions of China" 12 (12): 2321-2323, 2013