Tyrosinase 하향조절을 통한 대두(Glycine max (L.) Merr) 추출물의 멜라닌 생성 억제 효과 = Inhibitory Effect of Glycine max (L.) Merr Extract on Melanogenesis through the Downregulation of Tyrosinase Expression

다국어 초록 (Multilingual Abstract)

To investigate the molecular mechanisms underlying the antimelanogenic effects of Glycine max (L.) Merr ethanol extract (GEE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, melanin content measurement, reverse transcription-polymerase chain reaction (RT-PCR), and cellular tyrosinase activity assays were performed using B16F10 melanoma cell models. Treatment with GEE at concentrations of 10, 50, 100, 200, and 400 μg/mL significantly inhibited melanin synthesis in a dose-dependent manner by 46.69%, 44.42%, 27.90%, 19.37%, and —3.15%, respectively, in B16F10 cells stimulated with 3-isobutyl-1-methylxanthine (IBMX). Furthermore, treatment with GEE downregulated tyrosinase gene expression and reduced cellular tyrosinase activity to 80.42%, 78.13%, 67.26%, 64.25%, and 40.46%, respectively, compared to the control. Specifically, GEE treatment at 400 μg/mL effectively suppressed melanogenesis by decreasing tyrosinase expression and directly inhibiting tyrosinase enzymatic activity in the B16F10 cells. Therefore, GEE may be a promising natural whitening agent with potential applications in skin health and for the prevention of pigmentation related disorders.