Long-Term Inorganic and Organic Fertilizations Affect CH4 Oxidation Potential and Methanotrophic Community Structure in Paddy Soils

다국어 초록 (Multilingual Abstract)

Long-term fertilizations of inorganic and organic fertilizers can affect microbial abundance and communitystructure in agricultural soils, particularly controlling the activities of methane (CH4) microbes such as methan otrophs in the rice paddy soils. However, the effect of fertilizations on CH4 oxidation potential and methan otrophic community remains unclear. In order to investigate the response of the soil bacterial communityabundance, composition, and CH4 oxidation potential under four different fertilization regimes (control, NPK,compost, and NPK + compost) in a 49-year old paddy field, the soil bacterial community abundance and struc ture including CH4-oxidizing microbes as well as the potential of CH4 oxidation were investigated by assessingreal time quantitative PCR (qPCR), Miseq illumina sequencing analysis based on 16S rRNA genes, and oxic invitro slurry incubation, respectively. Long-term fertilizations changed significantly soil biochemical character istics, mainly influencing carbon and nitrogen pools in rice paddy soils. CH4 oxidation potential was stimulatedby inorganic fertilizations, mainly increasing the relative abundance of the genus Methylosarcina probably dueto increased N availability and soil pH in the soils. Our results showed inorganic fertilizations may enhanceCH4 oxidation potential by altering methanotrophic communities, which potentially mitigate CH4 emissionsin paddy ecosystems during the cultivation. However, additional research would be necessary for a betterunderstanding of CH4 dynamics in rice paddy soils under the long-term field conditions.

참고문헌 (Reference)

1 FAO, "World fertilizer outlook and trends to 2020" Food and Agriculture Organization of the United Nations 2020

2 Ho, A., "Unexpected stimulation of soil methane uptake as emergent property of agricultural soils following bio-based residue application" 21 (21): 3864-3879, 2015

3 Schloss, P.D., "Toward a census of bacteria in soil" 2 (2): e92-, 2006

4 Zhao, J., "The pH-based ecological coherence of active canonical methanotrophs in paddy soils" 17 (17): 1451-1462, 2020

5 Saunois, M., "The global methane budget 2000-2017" 12 (12): 1561-1623, 2020

6 Seongwoo Choi ; 이주희 ; 이여명 ; 김필주 ; 조주식 ; 정용화 ; 임요섭 ; 김상윤, "The Effects of Ethephon Application on Suppressing Methane Emission and Stimulating Rice Productivity in a Rice Paddy Soil: A Pot Experiment" 한국토양비료학회 53 (53): 489-501, 2020

7 IPCC, "Synthesis report" Intergovernmental Panel on Climate Change 2014

8 Tripathi, B. M., "Soil pH mediates the balance between stochastic and deterministic assembly of bacteria" 12 (12): 1072-1083, 2018

9 Kolb, S., "Quantitative detection of methanotrophs in soil by novel pmoAtargeted real-time PCR assays" 69 : 2423-2429, 2003

10 Lauber, C. L., "Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale" 75 : 5111-5120, 2009

11 Le Mer, J., "Production, oxidation, emission and consumption of methane by soils: A review" 37 (37): 25-50, 2001

12 Frenzel, P., "Oxygen profiles and methane turnover in a flooded rice microcosm" 14 (14): 84-89, 1992

13 Bodelier, P.L.E., "Nitrogen as a regulatory factor of methane oxidation in soils and sediments" 47 (47): 265-277, 2004

14 Peng, S. -Z., "Nitrogen and phosphorus leaching losses from paddy fields with different water and nitrogen managements" 9 (9): 333-342, 2011

15 Liesack, W., "Microbiology of flooded rice paddies" 24 (24): 625-645, 2000

16 Conrad, R, "Microbial ecology of methanogens and methanotrophs" 96 : 1-63, 2007

17 Hoefman, S., "Methyloparacoccus murrellii gen nov., sp. nov., a novel methanotroph isolated from pond water in South Africa and Japan" 64 : 2100-2107, 2014

18 Hirayama, H., "Methylomarinovum caldicuralii gen. nov. sp. nov., a moderately thermophilic methanotroph isolated from a shallow submarine hydrothermal system, and proposal of the family Methylothermaceae fam. nov" 64 : 989-999, 2014

19 Wei, M., "Methane oxidation and response of Methylobacter/Methylosarcina methanotrophs in flooded rice soil amended with urea" 101 : 174-184, 2016

20 Chan, A.S.K., "Methane oxidation and production activity in soils from natural and agricultural ecosystems" 30 : 1896-1903, 2001

21 Lee, H. J., "Methane emission and dynamics of methanotrophic and methanogenic communities in a flooded rice field ecosystem" 88 (88): 195-212, 2014

22 Bodelier, P. L, "Interactions between nitrogenous fertilizers and methane cycling in wetland and upland soils" 3 (3): 379-388, 2011

23 Bodelier, P.L.E., "Interactions between methane and the nitrogen cycle in light of climate change" 9-10 (9-10): 26-36, 2014

24 Oremland, R. S., "Importance of methane-oxidizing bacteria in the methane budget as revealed by the use of a specific inhibitor" 356 (356): 421-423, 1992

25 이여명 ; 이시은 ; 이주희 ; Seongwoo Choi ; 김상윤, "Impacts of Different Nitrogen Fertilization on Greenhouse Gas Emissions and Lettuce Productivity in Upland Soils during Cultivation" 한국토양비료학회 53 (53): 600-613, 2020

26 Gutierrez, J., "Effect of rice cultivar on CH4 emissions and productivity in Korean paddy soil" 146 : 16-24, 2013

27 Roy, R., "Effect of methanogenic precursors(acetate, hydrogen, propionate)on the suppression of methane production by nitrate in anoxic rice field soil" 28 (28): 49-61, 1999

28 Wu, M., "Effect of long-term fertilization on bacterial composition in rice paddy soil" 47 (47): 397-405, 2011

29 이창훈 ; 강성수 ; 정기열 ; 김필주, "Effect of Long Term Fertilization on Microbial Biomass, Enzyme Activities, and Community Structure in Rice Paddy Soil" 한국토양비료학회 46 (46): 487-493, 2013

30 김상윤 ; Ju Hee An ; Kyung Hoon Park ; Sang Yeob Lee ; Hang-Yeon Weon ; Mee Kyung Sang ; Ju-Hee Lee ; Jaekyeong Song, "Effect of Bacillus CC112 Inoculation on Fungal Pathogens and Soil Microbial Community in a Ginseng-Cultivated Soil" 한국토양비료학회 53 (53): 128-139, 2020

31 Jae-Hyung Ahn ; Shin Ae Lee ; Jeong Myeong Kim ; Myung-Sook Kim ; Jaekyeong Song ; Hang-Yeon Weon, "Dynamics of bacterial communities in rice field soils as affected by different long-term fertilization practices" 한국미생물학회 54 (54): 724-731, 2016

32 Kim, G. W., "Different responses of nitrogen fertilization on methane emission in rice plant included and excluded soils during cropping season" 230 : 162-168, 2016

33 Kim, S. Y., "Combined effects of carbon, nitrogen and phosphorus on CH4 production and denitrification in wetland sediments" 259-260 : 354-361, 2015

34 Deutzmann, J. S., "Characterization and phylogeny of a novel methanotroph, Methyloglobulus morosus gen. nov., spec. nov" 37 : 165-169, 2014

35 이여명 ; 최성우 ; 이주희 ; 안혜린 ; 이창훈 ; 이평호 ; 정승택 ; 김상윤, "Characteristics of Distribution and Decomposition of Organic Matter in Soils Cultivated with Various Fruits and Vegetables in Plastic Film House Fields" 한국토양비료학회 54 (54): 401-412, 2021

36 Ho, A., "Biotic interactions in microbial communities as modulators of biogeochemical processes : Methanotrophy as a model system" 7 : 1285-, 2016

37 Fadrosh, D. W., "An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform" 2 (2): 6-, 2014