담배에 형질발현된 완두 rbcS GENE의 발현 = Expression pattern of transformed pea rbcS gene in tobacco

다국어 초록 (Multilingual Abstract)

Using a binary vector system with pea rbcS gene and hygromycin phosphotransferase gene for selectable marker, tobacco plant was transformed by means of a leaf disc transformation method. After co-cultivation, bacterial cells were removed by cefotaxim treatment. Transformed shoots were developed from the co-cultivated leaf disc within 1 to 2 weeks in selection media supplemented with 25㎍/㎖ hygromycin and 50㎍/㎖ kanamycin. While normal plants could not grow in the medium with 25㎍/㎖ hygromycin the transformed plants could survive in the medium with 200㎍/㎖ hygromycin. It meant that resistant gene and rbcS gene was expressed in regenerated tobacco plants. Differential protein accumulation of RUBISCO small subunit was not detected by SDS-PAGE.