Magnesium ascorbyl phosphate regulates the expression of inflammatory biomarkers in cultured sebocytes = Magnesium ascorbyl phosphate regulates the expression of inflammatory biomarkers in cultured sebocytes

다국어 초록 (Multilingual Abstract)

Background: Acne is a inflammatory skin disorders caused by inflammatory biomarkers. Magnesium ascorbyl phosphate (MAP) is a stable precursor of vitamin C. It achieves a constant delivery of vitamin C into the skin and has antioxidative effects. Objectives: To evaluate changes in the expression of inflammatory biomarkers after treatment of cultured sebocytes with MAP Methods: RT-PCR, ELISA and western blotting were performed for inflammatory cytokines, matrix metalloproteinases (MMPs), antimicrobial peptides (AMPs) and keratinocyte differentiation markers before and after treatment of cultured sebocytes with MAP (10-2 mol), lipopolysaccharide (LPS) and a combination of MAP and LPS. RT-PCR and western blotting were also done for Toll-like receptor-4 (TLR-4) and NF-ĸB before and after treatment of cultured sebocytes with MAP, LPS and a combination of MAP and LPS. Results: The expressions of inflammatory cytokines (e.g., IL-1 β and IL-8) and MMPs (e.g., MMP-9) were decreased after treatment with MAP and a combination of MAP and LPS, and increased after treatment with LPS. AMPs (e.g., LL-37) were decreased after treatment with MAP and a combination of MAP and LPS, and increased after treatment with LPS. The expression of TLR-4 and NF-ĸB was decreased after treatment with MAP and a combination of MAP and LPS, and increased after treatment with LPS. Conclusion: MAP, which is a stable precursor of vitamin C, may be a good effective and alternative agent to improve inflammatory reaction in acne.