정상인 및 인슐린 비의존성 당뇨병 (NIDDM)환자에서 고성능 인슐린 친화 칼럼을 이용한 적혈구 인슐린 수용체의 분리에 관한 연구 = Purification of Human RBC Insulin Receptor by High Performance Insulin Affinity Collumn

다국어 초록 (Multilingual Abstract)

Insulin receptor defect is thought to play a role in the pathogenesis of NIDDM. C-DNA cloning of insulin receptor has contributed to the identification of several structural defect of the insulin recepotr in patients with extreme insulin resistance. But the site of insulin receptor defect in NIDDM has not been determined unequivocally due to polymorphism in RFLP analysis of the insulin receptor gene. Also low yield of insulin reeptor protein through the purification process made it difficult to analysis the insulin receptor protein in NIDDM.
We purified RBC insulin receptor in normal control and NIDDM with the use of Con A-agarose column (Bio-Rad, USA) to get glycopeptide and high performance insulin affinity column to purify insulin receptor. 12II -insulin binding activity was evaluated in each fraction of the chromatogram, and the results were as follows.
1) RBC insulin receptor was concentrated more than 500 folds though Con A-agarose column.
2) RBC insulin receptor purified by Con A-agarose column was concentrated more than 10 folds through high performance insulin affinity column.
3) RBC insulin receptor was concentrated more than 5000 folds by Con A-agarose and high performance insulin affinity column, and the recovery of 125I -insulin binging activity was 60% in Con A-agarose and 90% in high performance insulin affinity column.
We suggest that such improvements in the yield of the purification of RBC insulin receptor will contribute to the future analysis of insulin receptor protein in NIDDM. (J Kor Soc Endocrinol 6:308~313, 1991)