腹腔渗出細胞와 血淸을 移入받은 햄스터에서 肝吸蟲 免疫의 移入 = Transfer of Immunity to Clonorchis sinensis in Golden Hamsters Given Peritoneal Exudate Cells and Serum

국문 초록 (Abstract)

肝吸蟲의 代謝産物로 感作한 햄스터의 腹腔渗出細胞와 그 血淸을 正常햄스터의 腹腔內에 注入함으로써 肝吸蟲에 대한 免疫의 移入與否를 究明하기 위해 donor햄스터로서는 近交系 golden hamster를 2群으로 나누어 한群에는 肝吸蟲 被襄幼蟲을 經口感染시켰고 다른 群에는 肝吸蟲 脫襄幼蟲을 腹腔內 注入한 다음 40日後에 麻醉死시켜 血淸과 腹腔渗出細胞를 採集하여 供試하였다.
recipient햄스터는 1群 3마리씩 6群으로 나누어 第1群은 肝吸蟲 被襄幼蟲을 經口感染시킨 햄스터의 腹腔渗出細胞를, 第2群은 이 脫襄幼蟲을 腹腔內注入한 햄스터의 腹腔渗出細胞를 各各 햄스터의 腹腔內注入하였고 第3群은 本被襄幼蟲을 經口感染시킨 햄스터의 血淸을, 第4群은 本脫襄幼蟲을 腹腔內注入한 햄스터의 血淸을 各各 햄스터의 腹腔內注入하였으며 第5群은 所謂 感染對照群으로서 肝吸蟲 被襄幼蟲 50마리를 經口感染시켰고 第6群은 非處置對照群으로 하였다.
第1次 感作한 4日後에 全햄스터에 本被襄幼蟲을 經口的 challenge感染시켜 隔日로 EpG를 計算하였고 約 40日後에 모두 屠殺하여 對照群의 EpG, worm burden 및 脾臟當 plaque形成 細胞數를 基準으로 하여 腹腔渗出細胞와 血淸에 의한 免疫의 移入與否를 判定하였다.
Challenge感染後 全햄스터에서 肝吸蟲卵이 formalin-ether 集卵法으로는 第17日째부터, Stoll氏蟲卵計算法으로는 第18日째부터 나타났으며, 第1, 第2, 第3 및 第4群에서 Eggs per Gram의 急激한 增加는 第31日째, 第5群(感染對照群)에서는 第33日째에 나타났는데 比하여 對照群에서는 第41日째에 나타났다.
worm burden은 第2群과 第4群 햄스터에서는 對照群에 比해 有意的 成蟲數의 減少를 나타내었으며, plaeuq形成 細胞는 第1群과 第2群 햄스터에서만 약간 檢出됨으로서 肝吸蟲의 經口感染과 脫襄幼蟲으로 感作된 腹腔渗出細胞를 腹腔 內에 注入받은 햄스터에서는 免疫이 移入되었으나 感作血淸의 腹腔內注入으로는 移入되지 않았다.

다국어 초록 (Multilingual Abstract)

In order to determine the effect of peritoneal exudate cells and sera in the transfer of immunity against the liver fluke, Clonorchis sinensis, the inbred golden hamsters were divided into 2 groups. One group was infected orally with the metacercariae of C. sinensis, and the other group was injected intraperitoneally with the excysted metacercariae of flukes.
Forty days after sensitization, the donor hamsters were killed by deep anesthesia and then the sera and peritoneal exudate cells were collected.
Recipient hamsters were divided into 6 equal groups for primary sensitization. The hamsters of Group 1 were injected intraperitoneally with peritoneal exudate cells of the donor hamsters infected orally, those of Group 2 were injected intraperitoneally with peritoneal exudate cells of the donor hamsters injected excysted metacercariae intraperitoneally, those of Group 3 were injected intraperitoneally with serum of the donor hamsters infected orally, and those of Group 4 were injected intraperitoneally with serum of donor hamsters injected excysted larvae intraperitoneally. Those of Group 5 were infected orally with 50 metacercariae of C. sinensis. The Group 6 hamsters served as controls.
Four days after the primary sensititization, recipient hamsters were challenged with 50 metacercariae, and Eggs per Gram(EpG) of fecal samples were counted from 15 to 43 days after challenge.
The recipients were killed about 40 days after challenge, and the transfer of immunity to the recipients was estimated by significant differences in EpG, mean worm burdens and plaque forming cells per spleen between experimental and control groups.
The eggs appeared in 17 days by the formalin-ether sedimentation and 18 days by Stoll's egg counting techniques. The sudden increases of EpG were noted in Groups 1, 2, 3 and 4(experimental groups), whereas it was 33 days in Group 5(infection control) and 41 days in Group 6(control).
The experimental hamsters(Group 2 and 4) harbored fewer worms than the control(Group 6), and the differences in mean worm burdens between Group 2 and Group 4 were significant.
However, the relatively small number of plaque forming cells were encountered only in Group 1 and Group 2.
It is likely that the transfer of peritoneal exudate cells from isogenic donor hamsters infected orally and the donors injected intraperitoneally with excysted metacercariae caused the expulsion of worms in the recipients.