체외수정 및 미세조작에 의한 가축배의 생산과 효율적 이용에 관한 연구 6. 소 핵이식배의 체외배양 , 동결보존 및 성판정에 관한 연구 = Studies on Production and Efficient Utilization of Livestock Embryos by In Vitro Fertilization and Micromanipulation 6. In Vitro Culture , Cryopreservation and Sexing of Nuclear Transplant Bovine Embryos

다국어 초록 (Multilingual Abstract)

This study was carried out to establish an effective method for the improvement of subsequent development after nuclear transfer(NT) in bovine embryo, for the extension of utility through freezing and sexing of NT embryos. The rates of cleaved and developed embryos upto morula-blastocyst stages after the NT in in vitro produced embryos were 60.6 and 3.7%, respectively. Whereas, in in vivo embryos, slightly higher developments were obtained(70.8 and 5.6%), respectively. No difference was found in embryonic development upto morulablastocyst stages among the different levels of EGF added to the serum containing medium. The rates of cleaved and developed embryos upto morula-blastocyst were higher when electrostimulation was performed either before fusion or after addition at 21h IVM. The survival rate of frozen NT embryos was higher in late morula than that of earlier embryos. PCR-based sexing of NT embryo demonstrated that higher proportion of embryos was male(1.9:1), instead of normal ratio(1:1).