제니핀에 의해 가교된 다공성 젤라틴/키토산 지지체의 조직공학적 특성 분석 = Preparation and Characterization of Genipin Crosslinked Gelatin/Chitosan Porous Scaffold for Tissue Engineering

다국어 초록 (Multilingual Abstract)

Blended natural polymers composed of β-chitosan (Cs) and gelatin (G) with various composition ratios were produced as candidate materials for biomedical application. Novel cross-linked porous scaffold compared of gelatin/chitosan 7:3 5:5 3:7 and different amounts of genipin concentration (0.001, 0.01 and 0.03 mg/ml). Genipin was used to crosslinking agent of gelatin/β-chitosan blends, promoting the formation of primary amine and tertiary amine between chitosan and gelatin, or by itself. The effects of composition and crosslinking on the physical-chemical properties of GCs composite scaffolds were evaluated by ATR-FTIR analysis, water uptake, degradation and in vitro cell viability test. The SEM analysis showed that the pore size of gelatin/chitosan porous scaffolds were increased with increasing blend ratio of chitosan. The ATR-FTIR showed that the amount of amine group of gelatin/ chitosan was decreased with increasing genipin concentration. As the genipin concentration in the scaffold decreased resulting in an enhanced water uptake ability. In this study, NIH3T3 cells were seeded gelatin/β-chitosan porous scaffolds for the cell cytotoxicity, cell proliferation. The porous scaffolds did not affect cell growth because of low cytotoxicity. The porous cross-linked scaffold with the improved characterization and cell viability may be suitable as a biocompatable scaffold for tissue engineering.

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