호도약침(胡桃藥鍼)이 수은(水銀)에 의한 급성신부전(急性腎不全) 가토(家兎)의 신세뇨관(腎細尿管) 물질이동계장애(物質移動系障碍)에 미치는 영향(影響) = The Effect of Juglandis Semen Herbal Acupuncture on Alterations of Tubular Transport Function in Rabbits with Mercury-Induced Acute Renal Failure

다국어 초록 (Multilingual Abstract)

Objective: This study was undertaken to determine if Juglandis Semen herbal acupuncture (JSA) exerts protective effect against alterations in membrane transport function in rabbits with mercury-induced acute renal failure. Methods: Nephrotoxicity was induced by subcutaneous administration of Hg(a single dose of 10 mg/kg) and JSA was performed at both sides of Shenshu(BL23, Sinsu) for 7 days. Results: The administration of Hg at a subcutaneous single dose of 10 mg/kg caused a reduction in GFR to 12% of the basal value and an increase in fractional Na+ excretion to 8.9-fold, indicating generation of acute renal failure. When JSA were given for 7 days prior to Hg administration, such changes were significantly attenuated. The fractional excretion of glucose and phosphate was increased to approximately 102- and 35-fold, respectively, in rabbits treated with Hg alone. The increase in rabbits treated with Hg following JSA are significantly Lower than that in animals treated with Hg alone. Uptakes of glucose and phosphate in purified isolated brush-border membrane and Na+-K+-ATPase activity in microsomal fraction were inhibited in rabbits treated with Hg alone, suggesting that impairment in proximal reabsorption of glucose and phosphate is resulted from direct damage of membrane transport carriers and disruption of the normal Na+ gradient. Such changes were prevented by JSA. Conclusion: These results indicate that the administration of Hg causes impairment in reabsorption of solutes in the proximal tubule via the generation of reactive oxygen species. JSA provides the protection against the Hg-induced impairment in proximal reabsorption, and its effect may resulted from its antioxidant action.