Enhanced Saccharification of Rice Straw Using Hypochloritehydrogen Peroxide

다국어 초록 (Multilingual Abstract)

Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B)solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, endoglucanase,hemicellulase, and β-glucosidase Accellerase 1000™ (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8mg of D-glucose and 224.0 mg of D-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L D-glucose and 4.49 g/L D-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%.

참고문헌 (Reference)

1 DiGuiseppi, J., "The toxicology of molecular oxygen" 12 : 315-342, 1984

2 Yue, Z. B., "Surfactant-enhanced anaerobic acidogenesis of Canna indica L. by rumen cultures" 99 : 3418-3423, 2008

3 Lee, Y. J., "Sugarcane bagasse oxidation using a combination of hypochlorite and peroxide" ELSEVIER SCI LTD 100 (100): 935-941, 2009

4 Ma, L., "Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidationimpaired mutant of Pseudomonas putida KT2442" 100 : 4891-4894, 2009

5 Eggeman, T., "Process and economic analysis of pretreatment technologies" 96 : 2019-2025, 2005

6 Kuhar, S., "Pretreatment of lignocellulosic material with fungi capable of higher lignin degradation and lower carbohydrate degradation improves substrate acid hydrolysis and the eventual conversion to ethanol" 54 : 305-313, 2008

7 Teymouri, F., "Optimization of the ammonia fiber explosion (AFEX) treatment parameters for enzymatic hydrolysis of corn stover" 96 : 2014-2018, 2005

8 Awafo, V. A., "Optimization of ethanol production by Saccharomyces cerevisiae (ATCC 60868) and Pichia stipitis Y-7124: A response surface model for simultaneous hydrolysis and fermentation of wheat strawOptimization of ethanol production by Saccharomyces cerevisiae (ATCC 60868) and Pichia stipitis Y-7124: A response surface model for simultaneous hydrolysis and fermentation of wheat straw" 22 : 489-509, 1998

9 "Optimization of alkaline protease production by batch culture of Bacillus sp RKY3 through Plackett-Burman and response surface methodological approaches" 99 (99): 2242-2249, 200805

10 Box, G. E., "On the experimental attainment of Optimum conditions" 13 : 1-45, 1951

11 Lee, J. H., "Modification of starch using dextransucrase and characterization of the modified starch" 26 : 143-150, 1998

12 Hamidimotlagh, R., "Mixed sugar fermentation by Pichia stipitis, Sacharomyces cerevisiaea, and an isolated xylose-fermenting Kluyveromyces marxianus and their cocultures" 6 : 1110-1114, 2007

13 Louwrier, A., "Industrial products - the return to carbohydrate-based industries" 27 : 1-8, 1998

14 Pareek, S., "Hydrolysis of newspaper polysaccharides under sulfate reducing and methane producing conditions" 11 : 229-237, 2000

15 Sun, Y., "Hydrolysis of lignocellulosic materials for ethanol production: A review" 83 : 1-11, 2002

16 Saha, B. C., "Hemicellulose bioconversion" 30 : 279-291, 2003

17 Cardona, C. A., "Fuel ethanol production: Process design trends and integration opportunities" 98 : 2415-2457, 2007

18 Nigam, J. N., "Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis" 87 : 17-27, 2001

19 Kovacs, K., "Enzymatic hydrolysis of steam-pretreated lignocellulosic materials with Trichoderma atroviride enzymes produced in-house" 2 : 14-, 2009

20 David, C., "Enzymatic hydrolysis and bacterian hydrolysis - fermentation of eucalyptus wood pretreated with sodium hypochlorite" 27 : 1591-1595, 1985

21 Nigam, J. N., "Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate" 90 : 208-215, 2001

22 McGinnis, G. D., "Conversion of biomass into chemicals with high-temperature wet oxidation" 22 : 633-636, 1983

23 Laplace, J. M., "Combined alcoholic fermentation of D-xylose and D-glucose by four selected microbial strains: Process considerations in relation to ethanol tolerance" 13 : 445-450, 1991

24 Yoswathana, N., "Bioethanol production from rice straw" 1 : 26-31, 2010

25 Day, D. F., "Biocide compositions and related methods. US Patent 6,866,870"

26 Amartey, S., "An improvement in Pichia stipitis fermentation of acid-hydrolysed hemicellulose achieved by overliming (calcium hydroxide treatment) and strain adaptation" 12 : 281-283, 1996

27 Gould, J. M., "Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification" 26 : 46-52, 1984