마우스 골수세포의 중기염색체 분석 및 미소핵 검사를 이용한 새로운 피폭선량 평가법 개발 = Development of a Noble Dosimetry using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice

다국어 초록 (Multilingual Abstract)

Development of a Noble Dosimetry using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice
Purpose: The purpose of this study was to ascertain whether biological dosimetries using both chromosomal aberrations and micronuclei were reliable to assess biological effects of radiations. Materials and Methods: Individual two mice were irradiated with 0, 2, 5, 10, 15, 20, 25 Gy of Cs-137 gamma-rays. The numbers of chromosomal aberrations in metaphase spreads were scored with light microscope and we obtained the dose-response relationships with frequencies of chromosomal aberrations. As a same method, after individual two mice were irradiated with 0, 1, 2, 3, 4 Gy, we developed the dose-response relationships with micronuclei assay. Results: The frequencies of both chromosomal aberrations and micronuclei increased with dose (D), in a linear-quadratic manner. The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis and 0.0019, 0.0073, and 0.0506 for micronuclei assay. Conclusion: This dose-response relationships resulting from both metaphase analysis and micronuclei assay may be useful for evaluating biological effects of radiations.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 대상 및 방법
• 결과
• 고찰
• REFERENCES