한국재래산양 태자 및 신생양 중뇌에서 GFAP 면역반응세포의 분포에 관한 형태학적 연구 = Morphological Study of GFAP Immunoreactive Cells on Fetuses and Neonate Mesencephalon of Korean Native Goat

국문 초록 (Abstract)

한국재래산양의 임신 30, 45, 60, 90, 105, 120일령 태자 및 신생양의 중뇌를 대상으로 면역조직화학적 방법을 이용하여 GFAP 면역반응세포를 형태학적으로 연구하여 아래와 같은 결과를 얻었다.
1. GFAP 면역반응세포는 임신 45일령 태자에서부터 관찰할 수 있었으며, 이들은 무극형이었다.
2. 임신 60일령 태자에서는 뭇극형 면역반응세포가 관찰되었으며, 임신 90일령 태자에서는 중뇌수도관주위구역에 뭇극형면역반응세포가 관찰되었다.
3. 방사형아교세포의 돌기형태의 변화는 무극형면역반응세포가 두극형세포로 변화하였고, 두극형세포는 홑극형으로 변한 다음 뭇극형면역반응세포로 변형되고 있었다.
4. 면역반응을 나타낸 두극형면역반응세포들의 돌기들은 뇌실에서 연질막으로 뻗고 있었으며 연질막부위에서 종말발로
확장되어 있었다.
5. 한 시야당 GFAP 면역반응세포의 수는 임신 45일령 태자에서 임신 90일령 태자까지는 증가하였고, 임신 90일령 태자
에서 105일령 태자까지는 감소하였으며 임신 120일령 태자에서는 그 수가 105일령 태자보다 약간 증가한 양상을 나타내었다.
6. GFAP 면역반응세포의 면역반응은 임신 95일령 태자에서 120일령 태자까지 감소하는 양상이 관찰되었지만 대부분의 연질막부위와 뇌실부위에서는 강한 면역반응을 나타내고 있었다.
이러한 결과들은 면역반응세포가 중뇌수도관주위구역보다 중뇌부위에서 더 빠르게 발달하고 있었다.

다국어 초록 (Multilingual Abstract)

GFAP (Glial Fibrillary Acidic Protein) was one of the intermediate filament group and used as an astrocyte marker.
The numerous studies about GFAP immunoreactive cell’s distribution were investigated for fetus, neonate and aged brains. There are several reports about that GFAP immunoreactive cells were appeared at early fetus and after birth.
In cases of mammalian fetus radial glia cells migrated toward pial surface at early stage and revealed GFAP
immunoreactivity by the immunostain. But in cases of rodents, they migrated at late gestation or after birth.
This study, the GFAP immunoreactive cells’ localizations and distribution in the fetuses (the 30 th, 45 th, 60 th, 90 th, 105 th, 120 th of gestation) and neonate mesencephalon of korean native goat were investigated by immunohistochemistry (ABC method).
The results obtained in this study were summarized as followings;
1. Multipolar astrocytes at 60 days of gestation were found in midbrain, in 90 days of gestation were found in cerebral aqueduct.
2. Radial glial cell presented 60 days of gestation and process of GFAP immunoreaction was to stretch out from
ventricular to pia mater and nonpolar immunoreactive cell was transformed to bipolar, monopolar and multipolar
immunoreactive cell.
3. The number of GFAP immunoreactive cells of field were gradually decreased from 90 days of gestation till 105
days of gestation. But in 120 days of gestation and newborn were slightly increased.
4. Immunoreactivity of GFAP immunoreactive cells were gradually decreased from 95 days of gestation till 120 days of gestatioin.
These results were suggested that radial glial cell of midbrain developed very earlier than that of cerebral aqueduct.
However, cerebral aqueduct developed lately than that of midbrain, but faster developing than other.