간암 세포주 HepG2의 ROS생성과 AKT와 STAT3 신호 억제를 통한 연교의 항암 효능

다국어 초록 (Multilingual Abstract)

Abstract


Anti-cancer effect of Forsythiae Fructus through ROS generation and inhibition of AKT and STAT3 phosphorylation in HepG2 liver cancer cells

Cho, Sung- Su
Dept. of Korean Medicine
Graduate School
Kyung Hee University
Seoul, Korea
Supervised by Prof. Ko, Seong-Gyu, K.M.D., Ph.D.,MPH

Objective: In this study, the anti-caner effects of Forsythiae Fructus was investigated in HepG2 liver cancer cells. Also, the present study identified signaling pathways that induce cancer cell death by Forsythiae Fructus.

Methods: Cells were treated with Forsythiae Fructus for 24 h and cell viability was determined by MTT assay. Colony formation was detected by staining with crystal violet. Protein expression was measured by western blotting. ROS generation was determined by flow cytometry.

Results: Forsythiae Fructus inhibited HepG2 cell viability at 500μg/mL. And colony formation was significantly decreased when Forsythiae Fructus was treated. Although the apoptosis was not induced, Forsythiae Fructus suppressed protein activity related to cell proliferation, especially AKT and STAT3 protein. In addition, ROS generation increased in a dose dependent manner.

Conclusion: Forsythiae Fructus showed anti-cancer effect on HepG2 liver cancer cells. Thus, present study suggests that Forsythiae Fructus have a possibility as new treatment agent.

Key words: Forsythiae Fructus; HepG2; ROS; AKT; STAT3; anti-cancer

목차 (Table of Contents)

• I. 서 론 1
• II. 실험 재료 및 방법 3
• 1. 실험 재료 및 시료 제조 3
• 2. 세포 배양 및 증식 억제 측정 4
• 3. Crystal violet 염색 5
• 4. western blotting 6
• 5. 세포 사멸 분석 7
• 6. ROS 측정 8
• 7. 통계처리 9
• III. 결 과 10
• 1. 연교에 의한 세포 생존율 억제 효과 10
• 2. 연교의 세포 증식 억제 12
• 3. 연교의 세포 사멸 효과 14
• 4. 연교에 의한 세포 증식 조절 16
• 5. 연교에 의한 세포 증식 관련 메커니즘 18
• 6. 연교에 의한 ROS생성 유도 20
• IV. 고 찰 22
• V. 결 론 25
• 참 고 문 헌 26
• ABSTRACT 29