새로운 잠재혈흔 현출제로서 Diimine-type Ligand들의 잠재력 = The Potential of Diimine-type Ligands as New Latent Bloodstains Detectors

다국어 초록 (Multilingual Abstract)

To examine the potential of diimine-type ligands (L, 1-7) as new latent bloodstains detectors, the positive experimental results for blood were confirmed as stated below. The bubble phenomenon with the deoxygenation of bloodstain samples is an easy common method used to check latent bloodstains. We concluded that the modified experiment method was suitable when applied to the bloodstains detection by the ligands. The experimental results of the ligands were generally similar to that of the fluorescence phenomenon of 395nm (light yellowish green) and was clearer than those of 470nm (light brown) in a dark place. Not only that, the UV spectra of 1,10-phenanthroline molecule as bidentate ligand and its iron (II) complex ([Fe(L)_3)^(2+)]X^(2-)) with ferrous (Fe^(2+)) chloride as the model compound of hemoglobin were discussed. In the case of 1,10-phenanthroline, for example, the toxicity was known to be relatively low and the complex showed the fluorescence for a long time. Therefore, it can be regarded as a success that the results of the fluorescence phenomenon provide safe and convenient access to information on latent bloodstains from using the detectors.

참고문헌 (Reference)

1 임시근, "신속 FOB(분변 잠열) 검사 키트를 이용한 혈흔 검출 및 인혈 검사" 한국분석과학회 17 (17): 211-216, 2004

2 성낙도, "새로운 잠재혈흔 현출제의 조성물과 현출방법"

3 임채원, "범죄현장 혈흔검출 조성액"

4 WJ Bodziak, "Use of leucocrystal violet to enhance shoe prints in blood" 82 : 45-52, 1996

5 L Dilbeck, "Use of Bluestar forensic in Lieu of Luminol at crime scenes" 56 : 706-720, 2006

6 C Ling, L Song, "Tris(1,10-phenanthroline) iron(II)-μ-oxido-bis-[trichloridoferrate(III)]" 67 : 1232-1233, 2011

7 JW Stucki, "The quantitative assay of minerals for Fe2+ and Fe3+ using 1,10-phenanthroloine: II. A photochemical method" 45 : 638-641, 1981

8 JW Stucki, "The quantitative assay of minerals for Fe2+ and Fe3+ using 1,10-phenanthroline. I. Sources of variability" 45 : 633-637, 1981

9 PD Josephy, "The horseradish peroxidase-catalyzed oxidation of 3,5,3’,5’-tetramethylbenzidine; Free radical and charge-transfer complex intermediates" 257 : 3669-3675, 1982

10 RC Graham Jr, "The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique" 14 : 291-302, 1966

11 SK Lim, "Sensitivity and Specificity of Bloodstain Identification Reagents and their Effects on DNA Typing" 10 : 63-67, 2009

12 TA Brettell, "Review; Forensic science" 73 : 2735-2744, 2001

13 F Aguilar, "Re-evaluation tartrazin (E102): EFSA panel on food additives and nutrient sources added to food (ANS)" 7 : 331-1383, 2009

14 K Peter, "Quantitative assay of minerals for Fe2+ and Fe3+ using 1,10-phenanthroline: III. A rapoid photochemical method" 36 : 379-381, 1988

15 CW Lim, "Preliminary test of forensic identification bloodstain using Guaiac mixture and it's effect on genetic analysis" 42 : 22-31, 2010

16 LCAM. Bossers, "Methods for the enhancement of fingermarks in blood" 210 : 1-11, 2011

17 K Benkhedda, "Isotope ratio measurements of iron in blood samples by multi-collector ICP-MS to support nutritional investigations in humans" 122 : 179-192, 2008

18 F Barni, "Forensic application of the luminol reaction as a presumptive test for latent blood detection" 72 : 896-913, 2007

19 Allison N., "Forensic Biology Evidence Screening Past and Present. Canadian Soc" 42 : 101-120, 2009

20 CJ Frégeau, "Fingerprint enhancement revisited and the effects of blood enhancement chemicals on subsequent Profiler Plus™ fluorescent short tandem repeat DNA analysis of fresh and aged bloody fingerprints" 45 : 354-380, 2000

21 SS Tobe, "Evaluation of six presumptive tests for blood their specificity, sensitivity, and effect on high molecular weight DNA" 52 : 102-109, 2007

22 PS Brasterman, "Electronic absorption of iron (II) complex of 2,2'-bipyridine, 2,2'- bipyrimidine, 1,10-phenanthroline and 2,2':6'2''-terpyridine and their reduction products" 31 : 555-559, 1992

23 ND Park, "Dye-binding capacities of proteins using Coomassie blue G250 in an acidic solution" 25 : 248-251, 1982

24 RS Kaplan, "Determination of microgram quantities of protein in the presence of milligram levels lipid with amido black 10B" 150 : 97-104, 1985

25 DC Haris, "Determination of Iron with 1,10-Phenanthroline. u: Quantitative chemical analysis, 6th ed" WH Freeman & Company 258-709, 2003

26 T. Nieman, "Detection Based on Solution-Phase Chemiluminescence Systems, In Chemiluminescence and Photochemical Reaction Detection in Chromatography" VCH 99-123, 1989

27 T Bevel, "Bloodstain Pattern Analysis with an Introduction to Crime Scene Reconstruction, 3rd Ed. (Practical aspects of criminal & forensic investigations series)" CRC Press 2008

28 H Dugas, "Bioorganic Chemistry; A Chemical Approach to Enzyme Action. 3rd Ed., Chapter 6; Metal ions" Springer 407-120, 1996

29 MB Levin, "Benzidine test for blood" 21 : 358-362, 1954

30 R Cesareo, "Analysis of iron in blood by radioisotope X-ray fluorescence" 11 : 16-18, 1975

31 NA Dyson, "Analysis of human blood and liver tissue for copper, zinc and iron by the method of proton-induced X-ray emission analysis" 46 : 309-319, 1978

32 RS Higaki, "A study of the sensitivity and specificity of phenolphthalin as an indicator test for blood" 9 : 97-102, 1976

33 M Cox, "A study of the sensitivity and specificity of four presumptive tests for blood" 36 : 1503-1511, 1991

34 A Jafarian-Dehkordi, "A new spectrophotometric method for direct determination of iron (III) in serum" 16 : 76-82, 2008

35 "2-Tolidine"