Increased hGM-CSF Production and Secretion with Pluronic F-68 in TransgenicNicotiana tabacum Suspension Cell Cultures

다국어 초록 (Multilingual Abstract)

The effects of Pluronic F-68, a nonionic surfactant, on the production and secretion of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in a transgenic Nicotiana tabacum cell suspension culture were investigated in this study. The addition of Pluronic F-68 was shown to extend cell survival in the stationary phase, but had no influence on effective initial cell growth. With regard to production, it increased the level of extracellular hGM-CSF two-fold. This may be attributable not only to the enhanced expression level, but also to the improved permeability of the cell membrane due to the interaction between Pluronic F-68 and the cell membrane and cell wall. The effect of Pluronic F-68 on the production and secretion of hGM-CSF in a bioreactor was also evaluated. hGM-CSF production in the bioreactor after the addition of Pluronic F-68 proved more effective than in flask cultures.

참고문헌 (Reference)

1 Laouar, L, "Yeast responses to nonionic surfactants" 18 : 433-438, 1996

2 Chattopadhyay, D, "The protective effect of specific medium additives with respect to bubble rupture" 45 : 473-480, 1995

3 Metcalf,D, "The granulocyte-macrophage colony- stimulating factors. Science 229" 16-22, 1985

4 Bassetti, L, "Surfactant-induced non-lethal release of anthraquinones from suspension cultures of Morinda citrifolia" 39 : 149-155, 1995

5 Murhammer,D. W, "Sparged animal cell bioreactors mechanism of cell damage and Pluronic F-68 protection. Biotechnol. Prog. 6" 391-397, 1990

6 Kumar G. B. S, "Secretion of hepatitis B surface antigen in transformed tobacco cell suspension cultures" 27 : 927-932, 2005

7 Sharma, N, "Production and secretion of human interleukin-18 in transgenic tobacco cell suspension culture" 11 : 154-159, 2006

8 James E. A, "Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells" 19 : 131-138, 2000

9 Ma J. K-C, "Plant-derived pharmaceuticals . the road forward" 10 : 580-585, 2005

10 Hellwig, S, "Plant cell cultures for the production of recombinant proteins" 22 : 1415-1422, 2004

11 Ma J. K-C, "Molecular farming for new drugs and vaccines" 6 : 593-599, 2005

12 Huang, Y. L, "Kinetics and modeling of GM-CSF production by recombinant yeast in a three-phase fluidized bed bioreactor" 53 : 470-477, 1997

13 Qu, J, "Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Freaux suspension cultures" 10 : 155-161, 2005

14 Caldwell, "Influence of medium exchange schedules on metabolic and GM-CSF secretion rates of genetically engineered NIH-3T3 cells. Biotechnol. Prog. 7" 1-8, 1991

15 Jo S.-H, "High-yield production of functional human lactoferrin in transgenic cell cultures of Siberian ginseng (Acanthopanax senticosus)" 11 : 442-448, 2006

16 Shin Y.-J, "High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture" 82 : 778-783, 2003

17 Chen T.-L, "Expression of bioactive human interferongamma in transgenic rice cell suspension cultures" 13 : 499-510, 2004

18 Libby, "Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. DNA 6" 221-229, 1987

19 Palomares, L. A, "Evidence of Pluronic F-68 direct interaction with insect cells: impact on shear protection, recombinant protein, and baculovirus production" 26 : 324-331, 2000

20 Wang W, "Efficient elicitation of ginsenoside biosynthesis in cell cultures of Panax notoginseng by using selfchemically- synthesized jasmonates" 10 : 162-165, 2005

21 Kumar,V.,L. Laouar,M. R. Davey,B. J. Mulligan,and K. C. Lowe, "Effects of Pluronic F-68 on growth of transformed roots of Solanum dulcamara. Biotechnol. Lett. 12" 937-940, 1990

22 Hellung-Larsen P, "Effects of Pluronic F-68 on Tetrahymena cells: protection against chemical and physical stress and prolongation of survival under toxic conditions" 76 : 185-195, 2000

23 Joshi, L., "Bioprospecting in plants for engineered proteins" 8 : 223-226, 2005

24 Gomord, V, "Biopharmaceutical production in plants: problems, solutions and opportunities" 23 : 559-565, 2005

25 Choi J.-H, "Antitumor activity of cell suspension culture of green tea seed (Camellia sinensis L.)" 11 : 396-401, 2006

26 Sharma N, "A simple purification procedure of biologically active recombinant human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture" 9 : 423-427, 2004