라벤더 에탄올 추출물과 하이드로졸의 항산화 및 항균 활성

다국어 초록 (Multilingual Abstract)

Lavender (Lavandula angustifolia Mill) is a type of dicotyledonous plant Labiatae (Lamiaceae) that is native to the Mediterranean. It is one of the commonly known herb types and is also called the queen of herbs, with over 30 known species. The main bioactive substances are linalool, linalyl acetate, ladanein, and apigenin, and they have soothing and antibacterial effects, so they are widely used in cosmetics, food and beverages, etc., and are used as folk remedies such as aromatherapy.
First, antioxidant and antibacterial activities were analyzed on different types of lavender (L. angustifolia, L. dentata, L. stoechas, L. spp.) using 80% EtOH extraction and hot water extraction method which is the most commonly used. The five species of lavender were selected from among the lavenders we commonly see and are used for ornamental purposes, edible purposes, and aromatherapy. Marino lavender (L. angustifolia), Sweet lavender (L. spp.) is used as ornamental lavender. French lavender (L. stoechas) as edible lavender, and aromatherapy lavender as True lavender (L. angustifolia) and Fringed lavender (L. dentata) were selected, and 5-year-old lavender stems were harvested from Wonpyeong Herb Farm located in Maesong-myeon, Hwaseong-si, Gyeonggi-do. After drying with warm air, the ground samples were extracted by placing them in 80% ethanol. For hot water extraction, a copper distillation machine was used.
To increase the content of functional substances in Ture lavender (L. angustifolia) from two different regions, two types of elicitors (MeJA, SA) were treated at different concentrations for 14 days and then subjected to hot water extraction and analysis.
Among the five types of lavender, Sweet lavender (L. spp.), which belongs to the shrub type, showed a large difference in leaf size at 1.69 cm compared to other species, and the dry matter rate was 25.46% and 24.87% in Ture lavender and Marino lavender, which are Lavandula angustifolia species, respectively. showed the highest result, and the chlorophyll content was also confirmed to be the highest in the two lavenders at 40.91 and 45.27.
Antioxidant analysis results TPC results analyzed five different types of lavender through hot water extraction and 80% ethanol extraction. Friged lavender showed the highest values of 9.06 µg GAE/mL and 8.30 µg GAE/mL, respectively, for both 80% ethanol extraction and hot water extraction. As a result of TFC analysis, it was not detected in hot water extraction, and when extracted with 80% ethanol, True Lavender showed the highest result of 59.75 µg/mL. DPPH was highest at 59.33% in true lavender in 80% ethanol extraction and 9.37% in Fringed lavender in hot water extraction. As a result of FRAP analysis, 7.39 µg/mL was detected in Fringed lavender in 80% ethanol extraction and FRAP was detected in hot water extraction. It didn't work. As a result of ABTS, 80% ethanol extraction showed that French lavender was the highest at 98.23%. Based on these results, the antioxidant content measured during hot water extraction was low, so lavender from two regions was extracted and concentrated.
When Ture lavender (L. angustifolia) extractors from two different regions were treated at different concentrations for 14 days, the highest result in terms of leaf length was found in the untreated Wonpyeong lavender, with an average of 7.8 cm, and the highest result was found in leaf length. The area results also showed the highest results in untreated Wonpyeong lavender at 1cm, and the root weight and root length also showed the highest results at 32.7 cm and 75.03 g in untreated Wonpyeong lavender. As a result of chlorophyll measurement, there was no significant difference in the results of chlorophyll a in Wonpyeong lavender, but the total amount of chlorophyll b was highest at 2.46 mg·g−1 FW when treated with SA 1.5 mg∙L-1, and chlorophyll b was also found in Deokpyeong lavender. Although there was no significant difference in the value of a, it was confirmed that the chlorophyll b content in untreated lavender was the highest at 1.15 mg·g−1 FW. When comparing the extraction amount, Wonpyeong lavender yielded more extraction amount, and the same large extraction amount of 300 mL was confirmed in both regions at SA 0.5 mg∙L-1 concentration.
As a result of the antioxidant analysis, TPC results showed that when Deokpyeong lavender was treated with MeJA 0.5 mg∙L-1, the highest concentration was 8 µg GAE/mL, and the TFC content was not detected in the same way as in five other types of lavender hot water extracts. The DPPH result was the highest at 24.06% when Deokpyeong lavender was treated with unconcentrated SA 1.0 mg∙L-1, and the ABTS result was different from the DPPH result when Wonpyeong lavender was treated with concentrated MeJA 1.5 mg∙L-1. It was highest at 72.65%. As a result of the antibacterial analysis, the results of the antibacterial analysis of five different types of lavender and the antibacterial test of True lavender from two different regions showed no reaction. Because this is an extract, no reaction appears to have occurred. As a result of analyzing the moisture content of True lavender in the two regions, when treated with MeJA 1.5 mg∙L-1, the highest values were confirmed at 1.06% and 0.96% in Wonpyeong and Deokpyeong lavender, respectively. As a result of the microbial activity analysis, most of the active reactions were shown, but neither Wonpyeong nor Deokpyeong lavender treated with SA 1.0 mg∙L-1 showed any active reaction, and the results of Deokpyeong lavender treated with SA 0.5 mg∙L-1 also showed no active reaction.
Therefore, through the results of this experiment, there were various studies on the effect of elicit treatment on enhancing the content of secondary metabolites, but in this study, it is judged that there was relatively no difference in content due to hot water extraction in the areas where there was no significant difference. It is judged that a higher concentration must be extracted when using hydrosol, and future research on scents related to lavender extract is necessary.

목차 (Table of Contents)

• Ⅰ. 서 언 1
• Ⅱ. 재료 및 방법
• 2.1 식물재료 6
• 2.2 라벤더의 생장 8
• 2.2.1 라벤더의 생장환경 8
• 2.2.2 엽록소함량 측정 8
• 2.3 엘리시터 처리 9
• 2.3.1 Methyl jasmonate(MeJA) 농도의 영향 9
• 2.3.2 Salicylic acid(SA) 농도의 영향 9
• 2.4 추출물 제조 9
• 2.4.1 식물 에탄올 추출 9
• 2.4.2 Hydrosol 추출 및 수분함량 측정 10
• 2.5 항산화 분석 12
• 2.5.1 총 폴리페놀 및 총 폴라보노이드 함량 12
• 2.5.2 DPPH 라디컬 소거활성 13
• 2.5.3 FRAP 분석 13
• 2.5.4 ABTS 라디컬 소거활성 14
• 2.6 항균 활성 분석 15
• 2.7 통계처리 15
• Ⅲ. 결과 및 고찰
• 3.1 라벤더의 생장 16
• 3.1.1 라벤더 5종의 생장 16
• 3.1.2 두 지역의 Ture lavender 농도별 엘레시터 처리 후 생장 19
• 3.1.3 엽록소함량 22
• 3.2 두 지역의 Ture lavender 농도별 엘레시터 처리 후 hydrosol 23
• 3.2.1 Hydrosol 추출량 및 수분함량 측정 23
• 3.3 항산화 분석 27
• 3.3.1 총 폴리페놀 및 총 폴라보노이드 함량 27
• 3.3.2 DPPH 라디컬 소거활성 31
• 3.3.3 FRAP 분석 35
• 3.3.4 ABTS 라디컬 소거활성 36
• 3.4 항균 활성 분석 40
• Ⅳ. 결론 50
• Ⅴ. 인용문헌 51
• 적요 56