한우 체외수정란의 수정란이식에 관한 연구

다국어 초록 (Multilingual Abstract)

These studies were carried out to improve the reproductive efficiency through embryos transfer of Hanwoo IVM/IVF embryos. Following routine IVM/IVF procedure, Oocytes and zygotes were cultured for 40 to 44 h in CR1aa medium with BSA. Then 2 to 8-cell embryos were removed the cumulus cell and were cultured in CR1aa medium containing 10% fetal bovine serum and 2.5 mM taurine in 5% O2 and 5% CO2 at 38.5℃. The fresh and frozen thawed embryos of the morulae and blastocysts cultured for 6 to 9 days in vitro were transferred into recipients. The pregnancy rates of the blastocyst produced for 6, 7, 8, and 9 days in in vitro culture were 41.9, 48.6, 57.9 and 47.4%, respectively. In the developmental stage, pregnancy rates of early blastocysts (41.7%), blastocysts (57.6%) and expanded blastocysts (50.0%) were higher than that of morulae stage (00.0%). Fresh and frozen embryos on the pregnancy rates were 48.9 and 50.0%, respectively. These results indicate that the pregnancy rate after transfer were affected on embryonic stage of in vitro embryos and in vitro culture periods.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• 1. 한우 난포란의 체외성숙 및 수정
• 2. 체외수정란의 체외배양
• 3. 체외수정란의 동결보존
• 4. 한우 체외수정란의 이식
• 5. 임신감정
• Ⅲ. 결과
• 1. 체외수정란의 체외발육 및 체외배양일수에 따른 수태율
• 2. 체외수정란의 발육상태에 따른 수태율과 이식 위치에 따른 수태율
• 3. 신선 및 동결란에 따른 이식 후 수태율
• Ⅳ. 고찰
• Ⅴ. 적요
• Ⅵ. 인용문헌