Artemia 공급 단계에서 은어(Plecoglossus altivelis), 조피볼락 (Sebastes schlegeli), 감성돔(Acanthopagrus schlegeli) 및 넙치(Paralichthys olivaeus)의 소화효소 활성 = Comparative Study of Digestive Enzyme in the Artemia Feeding Step of the Sweet Fish Plecoglossus altivelis, Rock Fish Sebastes schlegeli, Black Seabream Acanthopagrus schlegeli, and Olive Flounder Paralichthys olivaeus

다국어 초록 (Multilingual Abstract)

We compared the nutritional requirements of whole larvae of the black seabream Acanthopagrus schlegeli, sweet fish Plecoglossus altivelis, olive flounder Paralichthys olivaeus and rock fish Sebastes schlegeli. The larvae were 20, 30, 14 and 5 DAH (or spawning) of black seabream, sweet fish, olive flounder and rock fish, respectively. Specific α-amylase activity (mU/mg protein) was highest (8,324.9 mU/mg protein) in rock fish larvae (P<0.05). Specific trypsin-like protease activity was highest (11,330.1 mU/mg protein) in black seabream larvae (P<0.05), which also exhibited the highest activity, 685.5 mU/mg dry weight (P<0.05). The specific activities per mg protein and mg dry weight of black seabream were the highest (187.4 mU/mg protein and 11.3 mU/mg dry weight, respectively) (P<0.05). A/P, P/L and A/L ratios of rock fish were 1.47, 90.3 and 133.1, respectively (P<0.05). We present here basic larval digestive enzymatic nutritional requirement data.

참고문헌 (Reference)

1 Robyt JF, "The β-amylases. In: Starch and its derivates" Academic Press 477-497, 1968

2 Hsu TL, "The relationship between feeding habits and digestive protease of some freshwater fishes" 18 : 45-53, 1979

3 Fish GR, "The comparative activity of some digestive enzymes in the alimentary canal of tilapia and perch" 15 (15): 161-178, 1960

4 Kitamikado M, "Studies on the digestive enzymes of rainbow-trout: II. Proteases" 26 : 685-690, 1996

5 Zambonino Infante JL, "Sea bass (Dicentrarchus labrax) larvae fed different Artemia rations: growth, pancreas enzymatic response and development of digestive functions" 139 : 129-138, 1996

6 SPSS Inc, "SPSS Base 10.0.7 for Window"

7 Walter HE., "Proteinases: methods with hemoglobin, casein and azocoll as substrates. In: Methods of Enzymatic Analysis, Vol. V" Verlag Chemie 270-277, 1984

8 Lowry OH, "Protein measurement with the Folin-phenol reagent" 193 : 265-275, 1951

9 Lee SM, "Optimum dietary protein level of ayu (Plecoglossus altivelis)" 12 : 145-153, 1999

10 Alliot E, "Nutritional requirements of sea bass (Dicentrachus labrax) and study of the protein and lipid rate in the diet" 10 : 22-24, 1974

11 Glass HJ, "Metabolism in marine flatfish: IV. Carbohydrate and protein digestion in Atlantic halibut (Hippoglossus hippoglossus L.)" 86 : 281-289, 1987

12 Suárez MD, "Metabolic effects of changes in the dietary protein: carbohydrate ratio in eel (Anguilla anguilla) and trout (Oncorhynchus mykiss)" 10 : 143-156, 2002

13 Kurtovic I, "Lipases from mammals and fishes" 17 : 18-40, 2009

14 Schmidt FH, "Lipase, photometric assay. In: Methods of enzymatic analysis vol 2" Academic Press 819-823, 1974

15 Kuz’mina VV, "Level of total proteolytic activity in some species of fish from the Volga barin" 30 : 25-35, 1990

16 Kvåle A, "Leaching properties of three different microparticulated diets and preference of the diets in cod (Gadus morhua L.) larvae" 251 : 402-415, 2006

17 Blier PU, "Is the growth rate of fish set by digestive enzymes or metabolic capacity of the tissues? Insight from transgenic coho salmon" 209 : 379-384, 2002

18 Hidalgo MC, "Feeding of the European eel Anguilla anguilla: I. Influence of dietary carbohydrate level" 105 : 165-169, 1993

19 Bai SC, "Evaluation of leather meal andmeat and bone meal as the fish meal replacer in Israeli cap diets" 10 : 153-161, 1997

20 Kolkovski S., "Digestive enzymes in fish larvae - Application and implication - a review" 200 : 181-201, 2001

21 Furné M, "Digestive enzyme acitivities in Ddriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comparative study" 250 : 391-398, 2005

22 Ribeiro L, "Development of digestive enzymes in larvae ofSolea senegalensis, Kaup 1858" 179 : 465-473, 1999

23 Kunitz M., "Crystalline soybean trypsin inhibitor: II General properties" 30 : 291-310, 1947

24 Bai SC, "Comparison of growth and body composition in olive flounder laevae (Paralichthys olivaceus) fed domestic experimental and imported commercial microparticulated diets" 10 : 87-95, 1997

25 Hidalgo MC, "Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities" 170 : 267-283, 1999

26 Nakagawa H, "Characterization of the digestive tract of wild ayu" 68 : 341-346, 2002

27 Moyano FJ, "Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata)" 15 : 121-130, 1996

28 Jeong KS., "Availability of soybean meal and suitable protein, energy level in different types of diet of red seabream (Pagurus major)" 5 : 9-17, 1992

29 Kolkovski S., "Advances in marine fish larvae diets. In: Advances en Nutrición Acuícola IX. IX Simposio Internacional de Nutrición Acuicola" Universidad Autónoma de Nuevo León 20-45, 2008