RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      SCIE SCOPUS KCI등재

      Caenorhabditis elegans 생체대체모델을 이용한 한국 영유아분변 유래 프로바이오틱스 균주의 in vivo 장 우점능 검토 = Rapid in vivo Colonization Screening of Probiotic Bacteria Isolated from Human Infants using Caenorhabditis elegans Surrogate Host

      한글로보기

      https://www.riss.kr/link?id=A104376358

      • 0

        상세조회
      • 0

        다운로드
      서지정보 열기
      • 내보내기
      • 내책장담기
      • 공유하기
      • 오류접수

      부가정보

      다국어 초록 (Multilingual Abstract)

      The ability of probiotics to adhere to the intestinal epithelium likely plays an important role in their colonization of the gastrointestinal tract. Here, we performed high-throughput screening (HTS) for suitable characteristics of potential probiotic bacteria using attachment and colonization ability through a C. elegans surrogate in vivo model. A total of 100 strains of lactic acid bacteria (LAB) isolated from infant feces were subjected to the colonization assay using C. elegans intestine. Based on colonization ability, we showed that nine isolates have a high attachment ability during whole experimental periods (up to 168 h), compared to Lactobacillus rhamnosus strain GG as a control. Also, through the use of an in vitro cell attachment model, nine isolates revealed highly binding activity to the mucus layer. Next, the selected 9 isolates were assayed for their survival ability when exposed to acidic and bile conditions as well as cholesterol reduction and the utilization of prebiotic substrates. As a result, the isolated nine strains were determined to be highly resistant to acid and bile conditions. In addition, they have significant activity for the reduction of cholesterol and utilization of several prebiotic substrates as a carbon source. Finally, the selected nine strains were identified by either L. rhamnosus or L. plantarum (4 strains for L. rhamnosus and 5 strains for L. plantarum, respectively). Taken together, we propose that the direct colonization of probiotics using C. elegans may be applicable to the rapid screening of valuable probiotic strains in vivo.
      번역하기

      The ability of probiotics to adhere to the intestinal epithelium likely plays an important role in their colonization of the gastrointestinal tract. Here, we performed high-throughput screening (HTS) for suitable characteristics of potential probiotic...

      The ability of probiotics to adhere to the intestinal epithelium likely plays an important role in their colonization of the gastrointestinal tract. Here, we performed high-throughput screening (HTS) for suitable characteristics of potential probiotic bacteria using attachment and colonization ability through a C. elegans surrogate in vivo model. A total of 100 strains of lactic acid bacteria (LAB) isolated from infant feces were subjected to the colonization assay using C. elegans intestine. Based on colonization ability, we showed that nine isolates have a high attachment ability during whole experimental periods (up to 168 h), compared to Lactobacillus rhamnosus strain GG as a control. Also, through the use of an in vitro cell attachment model, nine isolates revealed highly binding activity to the mucus layer. Next, the selected 9 isolates were assayed for their survival ability when exposed to acidic and bile conditions as well as cholesterol reduction and the utilization of prebiotic substrates. As a result, the isolated nine strains were determined to be highly resistant to acid and bile conditions. In addition, they have significant activity for the reduction of cholesterol and utilization of several prebiotic substrates as a carbon source. Finally, the selected nine strains were identified by either L. rhamnosus or L. plantarum (4 strains for L. rhamnosus and 5 strains for L. plantarum, respectively). Taken together, we propose that the direct colonization of probiotics using C. elegans may be applicable to the rapid screening of valuable probiotic strains in vivo.

      더보기

      참고문헌 (Reference)

      1 이승훈, "유아 분변에서 분리한 Lactobacillus johnsonii IDCC 9203의 잠재적 프로바이오틱 특성" 한국미생물·생명공학회 36 (36): 121-127, 2008

      2 Reid, G, "Use of Lactobacillus to prevent infection by pathogenic bacteria" 4 : 319-324, 2002

      3 Brenner, S., "The genetics of Caenorhabditis elegans" 77 : 71-94, 1974

      4 Azcarate-Peril, M. A., "Temporal gene expression and probiotic attributes of Lactobacillus acidophilus during growth in milk" 92 : 870-886, 2009

      5 Erkkilä, S., "Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use" 55 : 297-300, 2000

      6 Coconnier, M. H., "Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucussecreting cell lines in culture" 58 : 2034-2039, 1992

      7 Saarela, M., "Probiotic bacteria: Safety, functional and technological properties" 84 : 197-215, 2000

      8 Roberfroid, M., "Prebiotics: The concept revisited" 137 : 830-837, 2007

      9 Biedrzycka, E., "Prebiotic effectiveness of fructans of different degrees of polymerization" 15 : 170-175, 2004

      10 Razin, S., "Phospholipid and cholesterol uptake by mycoplasma cells and membranes" 598 : 628-640, 1980

      1 이승훈, "유아 분변에서 분리한 Lactobacillus johnsonii IDCC 9203의 잠재적 프로바이오틱 특성" 한국미생물·생명공학회 36 (36): 121-127, 2008

      2 Reid, G, "Use of Lactobacillus to prevent infection by pathogenic bacteria" 4 : 319-324, 2002

      3 Brenner, S., "The genetics of Caenorhabditis elegans" 77 : 71-94, 1974

      4 Azcarate-Peril, M. A., "Temporal gene expression and probiotic attributes of Lactobacillus acidophilus during growth in milk" 92 : 870-886, 2009

      5 Erkkilä, S., "Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use" 55 : 297-300, 2000

      6 Coconnier, M. H., "Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucussecreting cell lines in culture" 58 : 2034-2039, 1992

      7 Saarela, M., "Probiotic bacteria: Safety, functional and technological properties" 84 : 197-215, 2000

      8 Roberfroid, M., "Prebiotics: The concept revisited" 137 : 830-837, 2007

      9 Biedrzycka, E., "Prebiotic effectiveness of fructans of different degrees of polymerization" 15 : 170-175, 2004

      10 Razin, S., "Phospholipid and cholesterol uptake by mycoplasma cells and membranes" 598 : 628-640, 1980

      11 Kleesen, B., "Oligofructose and long-chain inulin: influence on the gut microbial ecology of rats associated with a human faecal flora" 86 : 291-300, 2001

      12 Matsumura, A., "New binding assay and preparative trial of cell-surface lectin from Lactobacillus acidophilus group lactic acid bacteria" 82 : 2525-2529, 1999

      13 Fang, H., "Modulation of humoral immune response through probiotic intake" 29 : 47-52, 2000

      14 Powell, J., "Models of Caenorhabditis elegans infection by bacterial and fungal pathogens. In: Innate Immunity" Humana Press 403-427, 2008

      15 Ann, E. Y., "Microencapsulation of Lactobacillus acidophilus ATCC 43121 with prebiotic substrates using a hybridisation system" 42 : 411-419, 2007

      16 Saulnier, D. M. A., "Mechanisms of probiosis and prebiosis: Considerations for enhanced functional foods" 20 : 135-141, 2009

      17 Kim, J., "Leuconostoc kimchii sp. nov., a new species from kimchi" 50 : 1915-1919, 2000

      18 Reid, G., "Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens" 34 : 344-351, 1988

      19 Dambekodi, P. C., "Incorporation of cholesterol into the cellular membrane of Bifidobacterium longum" 81 : 1818-1824, 1998

      20 Na-Kyoung Lee, "Identification and Probiotic Properties of actococcus lactis NK24 Isolated from Jeot-gal, a Korean Fermented Food" 한국식품과학회 13 (13): 411-416, 2004

      21 Sridevi, N., "Hypocholesteremic effect of bile salt hydrolase from Lactobacillus buchneri ATCC 4005" 42 : 516-520, 2009

      22 Salminen, S., "Functional food science and gastrointestinal physiology and function" 80 : 147-, 1998

      23 Irazoqui, J. E., "Evolution of host innate defence: Insights from Caenorhabditis elegans and primitive invertebrates" 10 : 47-58, 2010

      24 Rudel, L, "Determination of cholesterol using o-phthalaldehyde" 14 : 364-366, 1973

      25 Kirjavainen, P. V., "Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease" 32 : 1-7, 2001

      26 Kim, Y., "Characterization of the cholesterol-reducing activity in a cell-free supernatant of Lactobacillus acidophilus ATCC 43121" 72 : 1483-1490, 2008

      27 Boris, S., "Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate" 83 : 413-420, 1997

      28 Han, K. S., "Characterization and purification of bacteriocin produced by Lactobacillus acidophilus ATCC 4356" 11 : 531-536, 2002

      29 Oh, S., "Characteriza tion and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC" 83 : 2747-2752, 2000

      30 Kim, Y., "C. elegans immune conditioning with the probiotic bacterium Lactobacillus acidophilus NCFM enhances Gram-positive immune responses" 80 : 2600-2508, 2012

      31 Riddle, D. L, "C. elegans II" Cold Spring Harbor Laboratory Press 1997

      32 Mitsuoka, T., "Bifidobacteria and their role in human health" 6 : 263-267, 1990

      33 Gilliland, S., "Assimilation of cholesterol by Lactobacillus acidophilus" 49 : 377-381, 1985

      34 Ouwehand, A. C., "Assessment of adhesion properties of novel probiotic strains to human intestinal mucus" 64 : 119-126, 2001

      더보기

      분석정보

      View

      상세정보조회

      0

      Usage

      원문다운로드

      0

      대출신청

      0

      복사신청

      0

      EDDS신청

      0

      동일 주제 내 활용도 TOP

      더보기

      주제

      연도별 연구동향

      연도별 활용동향

      연관논문

      연구자 네트워크맵

      공동연구자 (7)

      유사연구자 (20) 활용도상위20명

      인용정보 인용지수 설명보기

      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2019-02-28 학술지명변경 외국어명 : Korean Journal for Food Science of Animal Resources -> Food Science of Animal Resources KCI등재
      2010-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2008-09-30 학술지명변경 외국어명 : 미등록 -> Korean Journal for Food Science of Animal Resources KCI등재
      2008-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2005-01-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      2004-01-01 평가 등재후보 1차 PASS (등재후보1차) KCI등재후보
      2002-01-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
      더보기

      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 0.65 0.33 0.58
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.53 0.52 0.745 0.11
      더보기

      이 자료와 함께 이용한 RISS 자료

      나만을 위한 추천자료

      해외이동버튼