생녹혈의 건조 및 안정화 = Drying and Stabilization of Deer Blood

다국어 초록 (Multilingual Abstract)

According to traditional oriental medicine, only non-coagulated native deer blood is said to be effective, and coagulated deer blood is ineffective. Thus, a drying and tablet-producing method for deer blood was developed to maintain its physiological and therapeutic activity, and so that after drying, it can be redissolved and protected from coagulation. Proteases such as trypsin, pepsin, chymotrypsin, and aminopeptidase were added to the deer blood indicating that it coagulated in an hour, as shown by the reference. Wax gourd extract, which is high in protease, was added to the blood resulting in anticoagulation for 31 hours. Also, additions of 1% EDTA, 0.38% sodium citrate, 0.16% calcium oxalate, 1.2% ethanol, and 0.006% heparin to the deer blood resulted in anticoagulation for 1 hour, 4 hours, 2 hours, 1 hour, and 31 hours, respectively. In an experiment using 0.19% sodium citrate plus 1% wax gourd extract, and 0.006% heparin plus 1% wax gourd extract, anticoagulation was maintained for up to 72 hours. However, since heparin can not be used in food, the deer blood tablet was made with the addition of 0.19% sodium citrate and 1% wax gourd extract, followed by freeze drying. The dissolution rate for the tablet manufactured in this manner was 96.7%. And the dissolution rates for spray-dried deer blood, vacuum-dried deer blood, and marketed deer blood tablets were 85%, 81%, and 25.5%, respectively. The composition of the tablet produced from the freeze-dried deer blood was 56.4% protein, 18.7% lactose, 1.2% amino acids, 1.0% glucose, 0.7% lipids, 180 ㎎/100 g of iron, 13 ㎎/100 g of potassium, 39.1 ㎎/100 g of calcium, 480 ㎎/100 g of sodium, 368 ㎎/100 g of chloride, each.

국문 초록 (Abstract)

한방에서는 굳은 녹혈은 약효가 없고, 굳지 않은 생녹혈만
약효가 있다고 하므로 녹혈의 응고를 방지하고, 건조하고 난
후도 물에 다시 녹아서 생리활성을 잃지 않는 녹혈 제조 방법을 개발하였다. 트립신, 펩신, 키모트립신, 아미노펩티다아제
등의 단백질 가수분해효소를 녹혈에 가한 결과, 아무것도 가하지 않은 것과 동일하게 1시간에 녹혈이 응고되었다. 단백질
가수분해효소를 함유한 동아추출액을 가한 것은 31시간 동안
항응고 작용을 나타냈다. 녹혈에 대하여 1% EDTA는 1시간,
0.38% 시트르산 나트륨은 4시간, 0.16% 수산칼슘은 2시간,
1.2% 에탄올은 1시간, 6 ㎎/100 ㎖ 헤파린은 31시간 항응고 작용을 나타냈다. 시트르산나트륨 0.19%+동아추출액 1%와 헤파린 0.006%+동아추출액 1%를 가한 것은 72시간까지 항응고
작용을 나타냈다. 그러나 헤파린은 식품에 사용할 수 없으므로 녹혈에 시트르산 나트륨 0.19%와 동아추출액 1%를 가하여 동결건조하고 타블렛을 제조하였다. 이렇게 만든 타블렛의 물에 녹는 복원률은 96.7%였다. 복원성은 분무건조한 녹혈은 85%, 감압건조한 녹혈은 81%, 시판 녹혈 타블렛은 25.5%였다.
제조한 녹혈 타블렛은 56.4%, 유당 18.7%, 아미노산 1.2%,
글루코오스 1.0%, 지방질 0.7%, 철 180 ㎎/100 g, 칼륨 13 ㎎
/100 g, 칼슘 39.1 ㎎/100 g, 나트륨 480 ㎎/100 g, 염소 368 ㎎
/100 g을 나타냈다.

참고문헌 (Reference)

1 안용근, "동아의 단백질가수분해효소" 한국식품영양학회 15 (15): 131-136, 2002

2 김한섭, "녹혈의 화학요법 항암제 부작용 억제 효능" 한국생약학회 34 (34): 145-149, 2003

3 KFIA, "Toxitic metal, Food code"

4 Park, DG, "The preparation method of the young antlers of the deer, deer blood or deer horns and the functional food"

5 Hwang, GS, "The characteristics of freeze-drying for deer blood" 5 : 69-83, 1991

6 Ministry of Agriculture and Forestry, "Statistics of the rest livestock" 230-239, 2007

7 Gu, BS, "Side effect by overusing the young antlers and blood of the deer" 256 : 94-95, 1999

8 Lee HS, "Preparation of health composition contain deer blood. Application of Kor. Patent. 13,064"

9 John V. Dacie, "Practical haematology, anticoagulation,fibrinolytic activity and antiplated aggregation" Korea medical book publisfher 399-414, 1999

10 KFIA, "Lipid - ether extraction Food code. 2000"

11 Park, KP, "Hemopoietic effects of deer blood on cyclophosphamide induced pernicious anemia" 29 : 283-292, 1998

12 Hong, SB, "Hemopoetic and immunophysiologic effect of deer blood in a cyclophosphamide-treated murine model" Gunkuk Uni 2001

13 Shin, NS, "Hematological and serum chemical values in Pere David's deer and wapiti" 11 : 471-478, 1994

14 KFIA, "Food additives code"

15 Kim, WG, "Fixed dose regimen of heparine administration with activated coagulation time during cardiopulmonary bypass" 31 : 867-872, 1998

16 Lee, JG, "Experimental Parasitology Veterinary and Parasite in Blood" Daehan Printing & Publishing 179-480, 1989

17 Kim, SM, "Effect of deer blood on aplastic anemia induced mouse" 10 : 127-135, 2004

18 Heo, J, "Deer. Dongibogam. 1613" Namsandang 1128-1129, 1969

19 Yang, YS, "Clinic Hematology, Anticoagulant" Daehakseorim 8-13, 2001

20 Ann, YG, "Biochemical Experiment, Somogyi-Nelson Method" Yangseogak 44-45, 2000

21 Ann, YG, "Biochemical Experiment, Lowry Method" Yangseogak 29-30, 2000

22 Ann, YG, "Biochemical Experiment, Ferrous" Yangseogak 74-75, 2000

23 Ann, YG, "Biochemical Experiment, Chloride" Yangseogak 76-77, 2000

24 Ann, YG, "Biochemical Experiment, Calcium" Yangseogak 72-73, 2000

25 "Biochemical Experiment, Amino Acid-Ninhydrin Method" Yangseogak 18-19, 2000

26 Joh, DB, "Attention matter in collection of the young antlers and blood" 60 : 144-145, 1983

27 Yong, TS, "Ahumanparasite, Toxoplasma gondii, Babesia microti, a parasitic insect examination" Jungmoongak 65-185, 2004

28 Baek, IB, "A process for preparing deer blood powder. Application of Kor. Patent. 794"

29 Lee, HS, "A process for preparing deer blood powder. Application of Kor. Patent. 13,064" 1991

30 Amore-pacific Co. Ltd, "A process for preparing deer blood powder and health composition contain freeze dried deer blood. Application of Kor. Patent. 15,553"

31 Kim, BY, "A literature study on anti cancer effect of a hardy orange, Polyporus umbellatus, Houttuynia cordata and deer blood" 11 : 17-29, 1986

32 Korea Deer Breed, "A compssition of the young antlers and blood of deer" 6 : 10-13, 1989

33 Amore-pacific Co. Ltd, "A composition for health that contains octacosanol and deer blood. Kor. Patent. 4,003"

34 Korea Deer Breeder Association, "A Buying and drinking reason of deer blood" Korea Deer Breeder association 82-84, 2001