한국 재래종 무 및 갯무의 RAPD 분석에 의한 유연관계 = Genetic Relationship by RAPD Analysis of Korean Wild Radish and Local Cultivars in Radish

국문 초록 (Abstract)

한국 무(Raphanus sativus)의 주요한 재래종 23종과 제주도의 남 북 지역 및 동남부 해안지대에서 수집한 ‘갯무’ 18종 등 41점의 특성을 조사하는 한편 그들의 유전적 유연관계를 RAPD로 구명코자 하였다. 주중, 근중, 엽중의 조사 결과 ‘진주대평’ 계와 ‘알타리무’를 제외한 기타 재래종 계의 주중과 근중이 각각 1.8과 1.3㎏ 이상으로 크고 근중은 전체 주중의 약 72-82% 정도였다. ‘알타리무’ 계는 주중과 근중이 각각 0.8과 0.6㎏ 정도로 작으면서도 근중이 전체 주중의 약 75% 정도였다. ‘갯무’는 수집 지가 다름에도 불구하고 공시된 18점이 모두 지상부와 지하부 형태에서 비슷하여 차이점을 찾기가 어려웠다. 그리고 주중과 근중이 자식약세가 일어난 자식계를 제외해도 0.4와 0.2㎏ 정도로 아주 작았으며 뿌리의 비중은 주중의 약 40-50% 정도였다. 선발된 10mer random primer 22개로 RAPD를 수행한 결과 polymorphic band가 전체 306개였으며 그것을 기초로 dendrogram을 그렸다. 재래종 중 진주대평 무 계열의 12점이 한 군으로, 그리고 다른 재래 무 계열 5점이 다른 한 군으로 구분되었다. ‘알타리무’ 군은 유연관계에 있어서 오히려 ‘갯무’의 남제주군과 구분되지 않을 정도였다. ‘갯무’는 먼저 북제주 지역의 6점과 동남부 해안 지대의 3점이 한 군으로 묶이면서 계통간 유전적 거리가 비교적 가깝게 나타났다. 남제주 지역의 5점은 북제주와 동남 해안지대에서 수집된 계통들과는 다른군으로 분류되었다. 전체적으로 보면 주중과 뿌리가 큰 재래종 17점과 주중과 뿌리가 작은 ‘알타리무’를 포함한 ‘갯무’ 군 24점이 각기 다른 2개 군으로 크게 나누어졌다.

다국어 초록 (Multilingual Abstract)

Morphological characteristics of 41 entries consisting of 23 local cultivars and 18 wild collections (Gaenmoo) in radish (Raphanus sativus), were observed on the field and the genetic relationships were investigated with the random amplified polymorphic DNA (RAPD) technique. The root of 12 local cultivars designated ‘Jinju-daepyoung’ and the 5 others except ‘Altari-moo’ was as big as more than 1.3 ㎏ and 72 to 82% in portion of the plant weight. In the case of 6 ‘Altari-moo’, the root was as small as 0.6 ㎏ and 75% in portion of the plant weight. The wild radishes named ‘Gaenmoo’ are very similar in the morphology, even though they were collected from quite different areas. Their roots were as small as around 0.2 ㎏ and less than 50% in portion of the plant weight. Twenty two primers presenting more than 8 polymorphic bands in the test of a local cultivar of the ‘Jinju-daepyoung’ group and a wild accession of the ‘Gaenmoo’ group were selected among 146 operon random primers prior to genetic relationship investigation. They were applied to 41 entries and a dendrogram was figured with the results obtained. Twenty three local cultivars were divided into 3 different groups, the ‘Jinju-daepyoung’ group of 12 entries with the big root, another group of 5 entries with the big root and the ‘Aaltari-moo’ group of 6 small rooted entries. The 18 wild ‘Gaenmoo’ were also divided into 3 groups according to the collected area of Jeju Island, such as southern group of 5 entries, northern group of 6 entries including 3 south-east coast entries and the one-generation-selfed group of 4 entries originating from 2 plants of 2 collections in the southern part of Jeju Island. The small rooted local cultivar ‘Aaltari-moo’ was much more similar to the wild ‘Ggaetmoo’ radish rather than the big rooted local cultivar in the genetic relationship. In conclusion, 41 entries were divided into 2 main groups according to the plant weight and the size of the root.

목차 (Table of Contents)

• Abstract
• 서언
• 재료 및 방법
• 결과 및 고찰
• 초록
• 인용문헌

참고문헌 (Reference)

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