RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단 = Detection of Soybean yellow common mosaic virus by RT-LAMP

다국어 초록 (Multilingual Abstract)

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been
occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in
soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing,
mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method
allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator
for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected
plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers
named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP,
products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green
I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP
showed the specificity for only SYCMV tested in this study.

목차 (Table of Contents)

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