자간전증 임신부의 혈청 및 태반에서의 Vascular endothelial growth factor (VEGF)에 관한 연구 = Serum Concentrations of Vascular Endothelial Growth Factor (VEGF) and placental VEGF expression in patients with preeclampsia

다국어 초록 (Multilingual Abstract)

Objective: The goal of this study was to compare serum concentrations of VEGF, placental growth facto r(PlGF), soluble VEGF receptor-1 (sVEGFR-1) and the expression of VEGF-A in placental tissue from preeclamptic pregnancy with normal pregnancy. Methods: From pregnant women with (n=46) and without (n=40) preeclampsia, maternal serum in third trimester and placental tissue at delivery were collected. The serum concentrations of VEGF, PlGF, and sVEGFR-1 were measured. The expression levels of VEGF-A protein in placenta were assessed using Western blot. Results: The concentrations of total VEGF, PlGF were significantly decreased and that of sVEGFR-1 was significantly increased in patients with preeclampsia. The expression of VEGF-A protein was lower in preeclamptic placenta than in control placenta, but there was no significant difference. Conclusion: The abnormality of angiogenic factors (VEGF, PlGF, sVEGFR-1) may be important in the development of pathophysiology of preeclampsia. An elevation of sVEGFR-1 may lead to suppression of VEGF and PlGF effects, and also the down-regulation of VEGF-A protein in placenta may result in the decreased maternal vascular adaptation to pregnancy.

참고문헌 (Reference)

1 조용균, "자간전증의 병인에 있어서 VEGF (vascular endothelial growth factor)가 혈관 내피세포에 미치는 영향." 45 (45): 1354-1359, 2002

2 오민정, "자간전증 환자에서 혈관내피 성장인자의 발현." 44 (44): 1877-1882, 2001

3 조용균, "자간전증 임신부의 Vascular endothelial growth factor (VEGF)의 혈청농도." 43 (43): 1967-1971, 2000

4 Yancopoulos G.D, "Vascular-specific growth factors and blood vessel formation." 407 : 242-248, 2000

5 Senger D, "Vascular permeability factor (VPF, VEGF) in tumor biology." 12 : 303-324, 1993

6 Shore V.H, "Vascular endothelial growth factor, placenta growth factor and their receptors in isolated human trophoblast." 18 : 657-665, 1997

7 Ferrara N, "VEGF: an update on biological and therapeutic aspects." 11 : 617-624, 2000

8 Cooper J.C, "VEGF mRNA levels in placentae from pregnancies complicated by preclampsia." 103 : 1191-1196, 1996

9 Sgamgati E, "VEGF expression in the placenta from pregnancies complicated by hypertensive disorders." 111 : 564-570, 2004

10 Simmons L.A, "Uteroplacental blood flow and placental vascular endothelial growth factor in normotensive and pre-eclapmtic pregnancy." 107 : 678-685, 2000

11 Redman C.W, "The pathogenesis of preeclampsia." 29 : 518-522, 2001

12 Page N.M, "The endocrinology of preeclampsia." 57 : 413-423, 2002

13 Lyall F, "Suppression of serum vascular endothelial growth factor immunoreactivity in normal pregnancy and in preeclampsia." 104 : 223-228, 1997

14 Cheng Z, "Study on association of vascular endothelial growth factor with the pathogenesis of pregnancy induced hypertension." 36 : 72-75, 2001

15 Luttun A, "Soluble VEGF receptor Flt1: the elusive preeclampsia factor discovered?" 111 : 600-602, 2003

16 Weeler T, "Serum levels of vascular endothelial growth factor are raised in preeclamptic versus normotensive pregnant women." 4 : 194A-, 1997

17 Reuvekamp A, "Selective deficit of angiogenic growth factors characterises pregnancies complicated by preeclampsia." 106 : 1019-1022, 1999

18 Lachmeijer A.M, "Searching for preeclampsia genes: the current position." 105 : 94-113, 2002

19 Roberts J.M, "Recent insights into the pathogenesis of preeclampsia." 23 : 359-372, 2002

20 Solomon C.G, "Preeclmapsia-searching for the cause." 350 (350): 641-642, 2004

21 Roberts J.M, "Preeclampsia: more than pregnancy induced hypertension." 341 : 1447-1451, 1993

22 Torry D.S, "Preeclampsia is associated with reduced serum levels of placental growth factor." 179 : 1539-1544, 1998

23 Verheul H, "Platelet: transporter of vascular endothelial growth factor." 3 : 2187-2190, 1997

24 Lyall F, "Placental expression of vascular endothelial growth factor in placentae from pregnancies complicated by pre-eclampsia and intrauterine growth restriction does not support placental hypoxia." 18 : 269-276, 1997

25 Conrad K.P, "Placental cytokines and the pathogenesis of preeclampsia." 37 : 240-249, 1997

26 Kingdom J.C, "Oxygen and placental vascular development." 474 : 259-275, 1999

27 Tsatsaris V, "Overexpression of the soluble vascular endithelial growth factor receptor in preeclamptic patients: pathophysiological conse- quences." 88 : 5555-5563, 2003

28 Saito S, "Increased T-helper-1-type immunity and decreased T- helper-2- type immunity in patients with preeclampsia." 41 : 297-306, 1999

29 Khalik A, "Hypoxia down-regulates placenta growth factor, whereas fetal growth restriction up-regulates placenta growth factor expression: molecular evidence for ‘placental hyperoxia’ in intrauterine growth restriction." 79 : 151-170, 1999

30 Ferrara N, "Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene." 380 : 439-442, 1996

31 Cao Y, "Heterodimers of placenta growth factor/vascular endothelial growth factor." 271 (271): 3154-3162, 1996

32 Thadhani R, "First trimester placental growth factor and soluble fms-like tyrosine kinase 1 and risk for preeclampsia." 89 (89): 770-775, 2004

33 Baker P.N, "Elevated serum levels of vascular endothelial growth factor in patients with preeclampsia." 86 : 815-821, 1995

34 Taylor R.N, "Circulating factors as markers and mediators of endothelial cell dysfunction in preeclampsia." 16 : 17-31, 1998

35 Levine R.J, "Circulating angiogenic factors and the risk of preeclampsia." 350 (350): 672-683, 2004

36 Tsatsaris V, "Circulating angiogenic factors and preeclampsia." 350 (350): 2003-2004, 2004

37 Tsatsaris V, "Circulating angiogenic factors and preeclampsia." 350 : 19-, 2004

38 Torry D.S, "Angiogenesis and the expression of vascular endothelial growth factor in endometrium and placenta." 37 : 21-29, 1997