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      이종 심장 판막 및 대혈관 이식편과 심낭에서 효과적인 탈세포화 방법에 관한 연구: 탈세포화의 최적화 = Study on an Effective Decellularization Technique for Cardiac Valve, Arterial Wall and Pericardium Xenographs: Optimization of Decellularization

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      https://www.riss.kr/link?id=A104650939

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      다국어 초록 (Multilingual Abstract)

      Background: We attempted to reproduce a previously reported method that is known to be effective for decellularization, and we sought to find the optimal condition for decellularization by introducing some modificationsto this method. Material and Method: Porcine semilunar valves, arterial walls and pericardium were processed for decellularization with using a variety of combinations and concentrations of decellularizing agents under different conditions of temperature, osmolarity and incubation time. The degree of decellularization and the preservation of the extracellular matrix wereevaluated by staining with hematoxylin and eosin and with alpha-Gal and DAPI in some of the decellularized tissues. Result: Decellularization was achieved in the specimens that were treated with sodium deoxycholate, sodium dodesyl sulfate, Triton X-100 and sodium dodesyl sulfate with Triton X-100 as single-step methods, and this was also achieved in the specimens that were treated with hypotonic solution → Triton X-100 → sodium dodesyl sulfate, sodium deoxycholate → hypotonic solution → sodium dodesyl sulfate, and hypotonic solution sodium dodesyl sulfate as multi-step methods. Conclusion: Considering the number and the amount of the chemicals that were used, the incubation time and the degree of damage to the extracellular matrix, a single-step method with sodium dodesyl sulfate and Triton X-100 and a multi-step method with hypotonic solution followed by sodium dodesyl sulfate were both relatively optimal methods for decellularization in this study.
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      Background: We attempted to reproduce a previously reported method that is known to be effective for decellularization, and we sought to find the optimal condition for decellularization by introducing some modificationsto this method. Material and Met...

      Background: We attempted to reproduce a previously reported method that is known to be effective for decellularization, and we sought to find the optimal condition for decellularization by introducing some modificationsto this method. Material and Method: Porcine semilunar valves, arterial walls and pericardium were processed for decellularization with using a variety of combinations and concentrations of decellularizing agents under different conditions of temperature, osmolarity and incubation time. The degree of decellularization and the preservation of the extracellular matrix wereevaluated by staining with hematoxylin and eosin and with alpha-Gal and DAPI in some of the decellularized tissues. Result: Decellularization was achieved in the specimens that were treated with sodium deoxycholate, sodium dodesyl sulfate, Triton X-100 and sodium dodesyl sulfate with Triton X-100 as single-step methods, and this was also achieved in the specimens that were treated with hypotonic solution → Triton X-100 → sodium dodesyl sulfate, sodium deoxycholate → hypotonic solution → sodium dodesyl sulfate, and hypotonic solution sodium dodesyl sulfate as multi-step methods. Conclusion: Considering the number and the amount of the chemicals that were used, the incubation time and the degree of damage to the extracellular matrix, a single-step method with sodium dodesyl sulfate and Triton X-100 and a multi-step method with hypotonic solution followed by sodium dodesyl sulfate were both relatively optimal methods for decellularization in this study.

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      참고문헌 (Reference)

      1 Booth C, "Tissue engineering of cardiac valve prostheses I: development and histological characterization of an acellular porcine scaffold" 11 : 457-462, 2002

      2 Galili U, "The α-gal epitope (Galα1-3Gal_1-4GlcNAc-R) in xenotransplantation" 83 : 557-563, 2001

      3 Vesely I, "The hybrid xenograft/ autograft bioprosthetic heart valve: in vivo evaluation of tissue extraction" 60 : 359-364, 1995

      4 Chen RN, "Process development of an accellular dermal matrix (ADM) for biomedical applications" 25 : 2679-2686, 2004

      5 Uchimura E, "Novel method of preparing acellular cardiovascular grafts by decellularization with poly (ethylene glycol)" 67 : 834-837, 2003

      6 Hirsch D, "Inhibition of calcification of glutaraldehyde pretreated porcine aortic valve cusps with sodium dodecyl sulfate: preincubation and controlled released studies" 27 : 1477-1484, 1993

      7 Hodde J, "Fibronectin peptides mediate HMEC adhesion to porcine-derived extracellular matrix" 23 : 1841-1848, 2002

      8 Hudson TW, "Engineering an improved acellular nerve graft via optimized chemical processing" 10 : 1343-1358, 2004

      9 Woods T, "Effectiveness of three extraction techniques in the development of a decellularized bone- anterior cruciate ligament-bone graft" 26 : 7339-7349, 2005

      10 Dahl SL, "Decellularized native and engineered arterial scaffolds for transplantation" 12 : 659-666, 2003

      1 Booth C, "Tissue engineering of cardiac valve prostheses I: development and histological characterization of an acellular porcine scaffold" 11 : 457-462, 2002

      2 Galili U, "The α-gal epitope (Galα1-3Gal_1-4GlcNAc-R) in xenotransplantation" 83 : 557-563, 2001

      3 Vesely I, "The hybrid xenograft/ autograft bioprosthetic heart valve: in vivo evaluation of tissue extraction" 60 : 359-364, 1995

      4 Chen RN, "Process development of an accellular dermal matrix (ADM) for biomedical applications" 25 : 2679-2686, 2004

      5 Uchimura E, "Novel method of preparing acellular cardiovascular grafts by decellularization with poly (ethylene glycol)" 67 : 834-837, 2003

      6 Hirsch D, "Inhibition of calcification of glutaraldehyde pretreated porcine aortic valve cusps with sodium dodecyl sulfate: preincubation and controlled released studies" 27 : 1477-1484, 1993

      7 Hodde J, "Fibronectin peptides mediate HMEC adhesion to porcine-derived extracellular matrix" 23 : 1841-1848, 2002

      8 Hudson TW, "Engineering an improved acellular nerve graft via optimized chemical processing" 10 : 1343-1358, 2004

      9 Woods T, "Effectiveness of three extraction techniques in the development of a decellularized bone- anterior cruciate ligament-bone graft" 26 : 7339-7349, 2005

      10 Dahl SL, "Decellularized native and engineered arterial scaffolds for transplantation" 12 : 659-666, 2003

      11 Rieder E, "Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells" 127 : 399-405, 2004

      12 Gilbert TW, "Decellularization of tissues and organs" 27 : 3675-3683, 2006

      13 Kasimir MT, "Comparison of different decellularization procedures of porcine heart valves" 26 : 421-427, 2003

      14 Wilcox HE, "Biocompatibility and recellularization potential of an acellular porcine heart valve matrix" 14 : 228-237, 2005

      15 Freytes DO, "Biaxial strength of multilaminated extracellular matrix scaffolds" 25 : 2353-2361, 2004

      16 Lin P, "Assessing porcine liver-derived biomatrix for hepatic tissue engineering" 10 : 1046-1053, 2004

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