흰쥐 샘뇌하수체 Folliculostellate cell에서의 Metallothionein-1, 2의 발현 = Expression of Metallothionein-1, 2 in Folliculostellate Cells of Rat Anterior Pituitary Gland

국문 초록 (Abstract)

Metallothionein (MT) family는 세포 내의 중금속 결합 단백질로서 아연, 구리 등의 대사에 관여하며 뇌하수체를 포함한 다양한 조직에 분포한다. 광학현미경과 공초점주사현미경(confocal microscope)을 이용하여 정상 흰쥐의 샘뇌하수체에서 MT-1, 2의 면역반응성을 동정하고, 내분비세포의 조절에 관여하는 folliculostellate cell이나 성장호르몬분비세포와의 연관성을 형태학적으로 관찰 하고자 이 연구를 시행한 결과 다음과 같은 결과를 얻었다. 흰쥐 뇌하수체 앞엽의 pars distalis와 pars intermedia에서 모두 MT-1, 2 면역반응이 관찰되었다. 공초점주사현미경을 이용하여 이중면역조직화학을 시행한 결과 MT-1, 2 면역반응이 S100 양성인 folliculostellate cell과 cyst-lining cell에 나타남을 확인하였으나, 성장호르몬분비세포에서는 MT-1, 2가 관찰되지 않았다. 이것은 샘뇌하수체의 pars distalis의 folliculostellate cell이 MT-1, 2를 함유한다는 최초의 보고이다. 이상의 결과를 종합하면 folliculostellate cell에 존재하는 MT-1, 2가 샘뇌하수체의 내분비작용과 관련이 있을 것으로 생각되며 또한 MT-1, 2가 folliculostellate cell에 특이적인 염색성을 보이는 것으로 미루어 folliculostellate cell의 새로운 표지물질로 사용될 수 있을 것으로 기대된다.

다국어 초록 (Multilingual Abstract)

Metallothionein (MT) family, intracellular zinc binding proteins, has been suggested to play an important regulatory role in zinc metabolism. The present study utilized light and confocal microscopic methods to investigate the expression pattern of MT-1, 2 and to determine the distribution and extent of colocalization of MT-1, 2 relative to subpopulations of S100 positive folliculostellate (FS) cells and somatotrophs in normal rat anterior pituitary. By light microscopy, MT-1, 2 immunoreactivity was observed both in pars intermedia and pars distalis of anterior pituitary.
Confocal microscopy revealed that most MT immunoreactivity was colocalized in S100 positive FS cells, not in
somatotrophs. This is the first report that FS cells in pars distalis contain MT-1, 2. These results suggest that MT family may be involved in regulation of hypophyseal endocrine functions and can be used as new markers of FS cells.

참고문헌 (Reference)

1 "abrain-specific member of the metallothionein gene family" 6333-6337, 1992

2 "Zinc in the anterior pituitary of rat" neuroendocri (neuroendocri): 233-242, 1987

3 "Trace metals in endocrinology" 779-797, 1976

4 "Trace elements in human physiology and pathology: zinc and metallothioneins" 57 : 399-411, 2003

5 "Selenium in the anterior pituitaryof rats exposed to sodium selenite" 67-74, 1985

6 "S100 protein in folliculostellatecells of the rat pituitary anterior lobe" 1980

7 "Roles of the metallothioneinfamily of proteins in the central nervous system" 55 (55): 133-145, 2001

8 "Pituitaryfolliculostellate cells immunostained with S100 protein antiserumin postnatal" 235-249, 1983

9 "Noval clonal strain from adultrat anterior pituitary producing S-100 protein" (gami h) : -303, 1983

10 "New perspectives in the functionof pituitary folliculo-stellate cells" 71 : 73-81, 1990

11 "New functional and structural insights" 4 : 177-183, 2000

12 "Marginal and folli-culostellate cells of the pituitary gland of the rat" 131 : 47-51, 1988

13 "Localization ofmetallothionein 1-2 immunoreactivity in bovine pituitary gland" 70 : 659-667, 2001

14 "Hiddenface of the anterior pituitary" 13 : 304-309, 2002

15 "Formation of mixedfollicles in anterior pituitary of perinatal rat-with special referenceto follicular growth hormone cells" 641-647, 1988

16 "Folliculo-stellate cellsand intercellular communication within the rat anterior pituitarygland" 39 : 138-149, 1997

17 "Evidence for functional communicationbetween folliculo-stellate cells and hormone-secreting cellsin perifused anterior pituitary cell aggregates" endocri (endocri): 685-691, 1987

18 "Encyclopedia of inorganic chemistry" Wiley 4 : 2229-2241, 1994

19 "Dome formation of keratin-containinggranular cells from rat anterior pituitary gland in vitro" 1193-1200, 1988

20 "Disruption of metallothionein gene in mice Analysis of brainzinc and neuron vulnerability to metals, aging and seizures" 17 : 1271-1281, 1997

21 "COMMENTARY -Metallothioneins in Brain- The Rolein Physiology and Pathology" 142 : 229-242, 1997

22 "Autocrine and paracrineroles of polypeptide growth factors, cytokines and vasogenic substances in normal and tumorous pituitary function and growth: a review" 135 : 515-532, 1996

23 "Analysis of S-100 protein positive folliculostellatecells in rat pituitary tissues" 338-346, 1988

24 "An Electron microscopic study on stellate cells in theirrabbit adenohypophysis under various endocrine conditions" 237-246, 1980

25 "A novel view of the function of pituitary folliculostellatecell network" 12 : 378-380, 2001