가계분석에 의한 k- casein 과 β- lactoglobulin 의 유전자형 분리에 관한 연구 = Studies on the Segregation of k- casein and β- lactoglobulin Genotypes in two Families of Dairy Cows

다국어 초록 (Multilingual Abstract)

This study was performed to offer basic data of both reducing generation interval and improving selection accuracy of dairy cattle for application of parentage test according to analyzing loci of κ-casein and β-lactoglobulin in related Korean dairy cattle using the PCR. The blood samples used in this study were taken from 24 related dairy cattle in Sin-Jung dairy farm and the frozen semen of two straws used in this study were taken from commercial firms. The results obtained were as follows; 1. This study established the extracting method of high quality DNA from the blood samples of 24 related dairy cattle and from two frozen semen samples by using phenol and determined the analyzing method for the polymorphism of κ-casein and β-lactoglobulin using PCR. 2. According to the restricted enzyme digestion in amplified products of κ-casein and β-lactoglobulin using PCR, κ-casein showed AA and AB types, and β-lactoglobulin showed AA, AB, and BB types, respectively. 3. By restricting enzyme digestion, genotypes of κ-caaein and β-lactoglobulin for dairy cows were always derived from genotypes of one parent or both parents. 4. Genetic panems between κ-casein and β-lactoglobulin markers showed independent gene segregations, therefore, in case of being known sire genotypes, genotypes of daughters would be detemuned by probability. 5. For ttte genetic improvement of dairy cattle, if the semen of sire with κ-casein BB or β-lactoglobulin BB were used by the A.I, genotyping method of marker genes can be useful for early identification and selection of dairy calves.